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WB: Acid extracts from untreated (lane 1) and sodium butyrate treated (lane 2) HeLa cells were probed with anti-acetyl-Histone H4 (Lys5)
Anti-acetyl-Histone H4 (Lys5)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Vrt, Yeast (S. cerevisiae) | Chromatin Immunoprecipitation (ChIP), WB, ChIP-seq | Rabbit | Serum | Polyclonal Antibody |
Description:
Anti-acetyl-Histone H4 (Lys5)
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes acetyl-histone H4 (Lys5), MW ~10 kDa.
Molecular Weight:
~10 kDa
Epitope:
N-terminus
Immunogen:
Synthetic peptide (SGRG[AcK]GGKGL-C) corresponding to amino acids 1-10 of yeast histone H4 acetylated on lysine 5, with a C-terminal cysteine added for conjugation purposes.
Modifications:
Acetylation
Isotype:
IgG
Background Information:
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
Species Reactivity:
- Human
- Vertebrates
- Yeast (S. cerevisiae)
Species Reactivity Note:
Yeast and human. Broad species cross-reactivity is expected.
Application Notes:
Chromatin Immunoprecipitation: 0.02-0.1 μL of this antibody immunoprecipitated chromatin from a wild type yeast strain, but not from a yeast strain containing a histone H4 K5R substitution.
Control:
Acid extracts from sodium butyrate treated HeLa cells.
Quality Assurance:
Evaluated by western blot on acid extracts from sodium butyrate treated HeLa cells.
Western Blot Analysis:
1:500-1:2000 dilutions of this antibody detected acetyl-histone H4 (Lys5) in acid extracts from HeLa cells, but did not detect unmodified histone H4 (Catalog # 14-412).
Western Blot Analysis:
1:500-1:2000 dilutions of this antibody detected acetyl-histone H4 (Lys5) in acid extracts from HeLa cells, but did not detect unmodified histone H4 (Catalog # 14-412).
Purification Method:
Unpurified
Presentation:
Rabbit polyclonal antiserum in buffer containing 0.05% sodium azide and 30% glycerol.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- HIST1H4A
- H4/J
- HIST1H4F
- HIST1H4L
- H4FN
- H4FA
- H4FH
- HIST1H4H
- H4F2
- H4FM H4/A H4FK
- H4FG
- HIST2H4
- H4FB
- H4/K
- HIST1H4I
- H4/N
- HIST1H4B
- H4FE
- H4/M
- H4/a
- HIST1H4E
- HIST4H4
- HIST2H4A
- H4FC
- H4FI
- H4/H
- HIST1H4C
- H4/G
- HIST1H4K
- H4FJ
- H4FD
- H4/I
- H4/B
- H4/D
- H4/C
- HIST1H4J
- HIST1H4D
- H4/E
Alternate Names:
- H4K5Ac
- Histone H4 (acetyl K5)
- H4 histone family, member A
- histone 1, H4a
- histone cluster 1, H4a
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Chromatin Immunoprecipitation (ChIP)
- Western Blotting
- ChIP-seq
Entrez Gene Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
UniProt Summary:
FUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 103 amino acids; 11367 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
SIZE: 103 amino acids; 11367 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
Brand Family:
Upstate
Product Name:
Anti-acetyl-Histone H4 (Lys5)
Concentration:
Variable
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µL
Format:
Serum
Host:
Rabbit