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WB: A431cell lysate was probed with Anti-AMP-Activated Protein Kinase (AMPK) α1. Lane 1: antibody alone. Lane 2: antibody incubated with immunogenic...
Anti-AMPK α1
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M | WB | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-AMPK α1
Trade Name:
Upstate (Millipore)
Specificity:
AMPKα1
Molecular Weight:
63 kDa
Immunogen:
KLH-conjugated synthetic peptide corresponding to amino acids 376-392 (C-ARHTLDELNPQKSKHQG-COOH) of human AMP-activated Protein Kinase α1.
Isotype:
IgG
Background Information:
AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kDa) and non-catalytic beta and gamma subunits. The Alpha subunit has at least two isoforms (alpha 1 and alpha 2) that differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity.
Species Reactivity:
- Human
- Rat
- Mouse
Species Reactivity Note:
Human, rat, and mouse.
Control:
A431cell lysate, or lysate of HeLa cells treated with heat shock
Included Positive Antigen Control: Catalog # 12-301, Non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Included Positive Antigen Control: Catalog # 12-301, Non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Quality Assurance:
Evaluated by immunoblot in RIPA lysates from non-stimulated A431 cells.
Western Blot Analysis:
0.5-2 μg/mL of this antibody detected AMPK α1 in RIPA lysates from non-stimulated A431 cells.
Western Blot Analysis:
0.5-2 μg/mL of this antibody detected AMPK α1 in RIPA lysates from non-stimulated A431 cells.
Purification Method:
Protein A chromatography
Presentation:
Protein A purified rabbit in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- PRKAA1
- MGC57364
- AMPK
- MGC33776
- AMPKa1
- AMPK1
Alternate Names:
- 5'-AMP-activated protein kinase, catalytic alpha-1 chain
- AMP -activate kinase alpha 1 subunit
- AMP-activated protein kinase, catalytic, alpha-1
- AMPK alpha 1
- AMPK alpha-1 chain
- protein kinase, AMP-activated, alpha 1 catalytic subunit
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
UniProt Summary:
FUNCTION: SwissProt: Q13131 # Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
COFACTOR: Magnesium.
SIZE: 550 amino acids; 62808 Da
SUBUNIT: Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits. Interacts with FNIP1.
SIMILARITY: SwissProt: Q13131 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. & Contains 1 protein kinase domain.
COFACTOR: Magnesium.
SIZE: 550 amino acids; 62808 Da
SUBUNIT: Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits. Interacts with FNIP1.
SIMILARITY: SwissProt: Q13131 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. & Contains 1 protein kinase domain.
Brand Family:
Upstate
Product Name:
Anti-AMPK α1
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
200 μg
Format:
Purified
Host:
Rabbit