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WB: rat liver preparation was probed with Anti-AMP-Activated Protein Kinase (AMPK) α2 (2μg/ml) (lane 2) and antibody was incubated with immunogenic pe...
Anti-AMPK α2
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-AMPK α2
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes AMP-Activated Protein Kinase alpha2 (AMPKα2).
Molecular Weight:
~63 kDa
Immunogen:
peptide corresponding to amino acids 353-367 (C-DDSAMHIPPGLKPHP) of human AMP-Activated Protein Kinase alpha2 (AMPKalpha2) catalytic subunit
Isotype:
IgG
Species Reactivity:
- Human
- Rat
Control:
Rat liver preparation
Quality Assurance:
routinely evaluated by immunoblot in a rat liver preparation
Purification Method:
Protein A purfied
Presentation:
Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- PRKAA2
- AMPK
- AMPK2
- PRKAA
Alternate Names:
AMP-Activated Protein Kinase; AMPK
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P54646 # Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
COFACTOR: Magnesium.
SIZE: 552 amino acids; 62320 Da
SUBUNIT: Heterotrimer of a catalytic subunit, a beta and a gamma non-catalytic subunits.
SIMILARITY: SwissProt: P54646 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. & Contains 1 protein kinase domain.
COFACTOR: Magnesium.
SIZE: 552 amino acids; 62320 Da
SUBUNIT: Heterotrimer of a catalytic subunit, a beta and a gamma non-catalytic subunits.
SIMILARITY: SwissProt: P54646 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. & Contains 1 protein kinase domain.
Qty/Pk:
200 μg
Brand Family:
Upstate
Format:
Purified
Host:
Rabbit
Product Name:
Anti-AMPK α2
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody