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WB: HeLa cell lysate was probed with anti-Ago 2 (0.5μg/ml). Arrow indicates Ago 2 (~100kDa).
Anti-Ago 2
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-Ago 2
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Ago 2, also known as eukaryotic translation initiation factor 2C, 2 (EIF2C 2), MGC3183 and Q10.
Molecular Weight:
100 kDa
Immunogen:
GST fusion protein corresponding to residues 7-48 of human argonaute 2 (Ago2).
Isotype:
IgG
Background Information:
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.
Provides endonuclease activity to RNA-induced silencing complexes (RISC). Cleaves siRNA/mRNA heteroduplexes bound to RISC. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi).
Provides endonuclease activity to RNA-induced silencing complexes (RISC). Cleaves siRNA/mRNA heteroduplexes bound to RISC. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi).
Species Reactivity:
Human
Control:
HeLa cell lysate, HL-60 cell lysate
Quality Assurance:
Routinely evaluated by immunoblot in RIPA and cytosolic lysates from HeLa cells.
Purification Method:
Protein A purfied
Presentation:
Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- EIF2C2
- AGO2
- Q10
- PPD
- MGC3183
- Argonaute-2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: Q9UKV8 # Provides endonuclease activity to RNA-induced silencing complexes (RISC). Cleaves siRNA/mRNA heteroduplexes bound to RISC. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi) (By similarity).
SIZE: 851 amino acids; 96799 Da
SUBUNIT: Interacts with DICER1 through its Piwi domain.
SUBCELLULAR LOCATION: Cytoplasm, P-body. Note=Cytoplasmic bodies.
DOMAIN:SwissProt: Q9UKV8 Piwi domain is thought to provide RNA cleavage activity with a mechanism similar to RNase H (By similarity).
SIMILARITY: Belongs to the argonaute family. & Contains 1 PAZ domain. & Contains 1 Piwi domain.
SIZE: 851 amino acids; 96799 Da
SUBUNIT: Interacts with DICER1 through its Piwi domain.
SUBCELLULAR LOCATION: Cytoplasm, P-body. Note=Cytoplasmic bodies.
DOMAIN:SwissProt: Q9UKV8 Piwi domain is thought to provide RNA cleavage activity with a mechanism similar to RNase H (By similarity).
SIMILARITY: Belongs to the argonaute family. & Contains 1 PAZ domain. & Contains 1 Piwi domain.
Qty/Pk:
200 μg
Brand Family:
Upstate
Format:
Purified
Host:
Rabbit
Product Name:
Anti-Ago 2
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody