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WB: 20 micrograms of HeLa cell lysate was probed with anti-Ago 1 (1μg/ml).
Anti-Ago 1
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IP, WB | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-Ago 1
Trade Name:
Upstate (Millipore)
Product Overview:
Description: Antibody recognizing the human Argonaute-1 protein, a component of the RNAi machinery.
Specificity:
Ago 1
Molecular Weight:
95-100kDa
Immunogen:
Fusion protein corresponding to residues 1-46 of human Ago 1.
Isotype:
IgG
Background Information:
Ago1 (Eukaryotic translation initiation factor 2C 1) belongs to the argonaute family and contains 1 PAZ and 1 Piwi domain. It is required for G1 arrest and mating in response to nitrogen starvation. It is also known as cell cycle control protein through it's regulation of cytokinesis and cell cycle checkpoints occurs downstream of dcr1. It is required, indirectly, for regulated hyperphosphorylation of cdc2.
Ago 1 also has a role in the RNA interference (RNAi) pathway which is important for heterochromatin formation and accurate chromosome segregation. It has been shown to be non-essential for siRNA-directed target RNA cleavage but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage.
Ago 1 also has a role in the RNA interference (RNAi) pathway which is important for heterochromatin formation and accurate chromosome segregation. It has been shown to be non-essential for siRNA-directed target RNA cleavage but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage.
Species Reactivity:
Human
Application Notes:
This antibody will immunoprecipitate RNAi complexes.
Control:
HeLa nuclear extract
Quality Assurance:
Routinely evaluated by immunoblot in HeLa whole cell RIPA or cytosolic extracts.
Purification Method:
Protein A purfied
Presentation:
70% storage buffer (0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide) and 30% glycerol
Storage Conditions:
2 years at -20°C
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- EIF2C1
- AGO1
- EIF2C
- Argonaute-1
- GERP95
- Q99
- DKFZp686M13167
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of closely related family members including argonaute 3, and argonaute 4.
Key Applications:
- Immunoprecipitation
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: Q9UL18 # Plays an important role in the eukaryotic peptide chain initiation process.
SIZE: 857 amino acids; 97214 Da
SIMILARITY: SwissProt: Q9UL18 ## Belongs to the argonaute family. & Contains 1 PAZ domain. & Contains 1 Piwi domain.
SIZE: 857 amino acids; 97214 Da
SIMILARITY: SwissProt: Q9UL18 ## Belongs to the argonaute family. & Contains 1 PAZ domain. & Contains 1 Piwi domain.
Qty/Pk:
200 µg
Brand Family:
Upstate
Format:
Purified
Host:
Rabbit
Product Name:
Anti-Ago 1
Antibody Type:
Polyclonal Antibody