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  • : 100 µL
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WB: Acid extracted proteins from HeLa cells (lane 1) and recombinant histone H3 (lane 2) (14-494) were probed with anti-dimethyl-Histone H3 (Lys23) (1...

Anti-dimethyl Histone H3 (Lys23) Antibody

Species Reactivity Key Applications Host Format Antibody Type
H WB, DB, Mplex Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-dimethyl Histone H3 (Lys23) Antibody
Promotional Text:
Trade Name:
Upstate (Millipore)
Specificity:
dimethyl Histone H3 (Lys23)
Molecular Weight:
17kDa
Immunogen:
peptide containing the sequence ATdimKAA corresponding to dimethylated lysine 23 of human histone H3.
Modifications:
Methylation
Isotype:
Species Reactivity:
Human
Species Reactivity Note:
Broad species cross-reactivity is expected
Quality Assurance:
routinely evaluated by immunoblot on acid extracted proteins from HeLa cells
Purification Method:
Immunoaffinity purified
Presentation:
0.2M Tris-glycine, pH 7.4, 0.15M NaCl and 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
1 year at -20°C
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • H3F3A
  • MGC87783
  • H3.3A
  • MGC87782
  • H3F3
  • H3.3B
  • H3F3B
Alternate Names:
  • H3K23me2
  • Histone H3 (di methyl K23)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • Western Blotting
  • Dot Blot
  • Multiplexing
Entrez Gene Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
UniProt Summary:
FUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 136 amino acids; 15508 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
Brand Family:
Upstate
Product Name:
Anti-dimethyl Histone H3 (Lys23) Antibody
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µL
Format:
Affinity Purified
Host:
Rabbit
Applications:
Use Anti-dimethyl Histone H3 (Lys23) Antibody (Rabbit Polyclonal Antibody) validated in WB, DB, Mplex to detect dimethyl Histone H3 (Lys23) also known as H3K23me2, Histone H3 (di methyl K23).

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