Content Loading...
Content Loading...
Product Images
ICC: CMXRos (a mitochondrial marker; 1:8,000 dilution; Molecular Probes; red staining) was added to U937 cells growing in suspension, which were then ...
IB: 40 micrograms of rat brain microsomal preparation was probed with anti-Thioredoxin Reductase 1 (1μg/ml)
Anti-Thioredoxin Reductase 1 (TrxR1)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB, IC | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Thioredoxin Reductase 1 (TrxR1)
Trade Name:
Upstate (Millipore)
Specificity:
thioredoxin reductase 1
Molecular Weight:
58kDa
Immunogen:
peptide corresponding to amino acids 137-150 (C-PHRIKATNNKGKEK) of human thioredoxin reductase 1
Isotype:
IgG
Species Reactivity:
- Human
- Rat
Quality Assurance:
routinely evaluated by immunoblot in RIPA lysates from rat brain microsomal preparation
Purification Method:
Immunoaffinity purified
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
1 year at -20°C
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- TXNRD1
- TXNR
- oxidoreductase
- TRXR1
- Trxr1
- MGC9145
- TR
- GRIM-12
- TR1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms.
Key Applications:
- Western Blotting
- Immunocytochemistry
UniProt Summary:
COFACTOR: Binds 1 FAD per subunit.
SIZE: 499 amino acids; 54707 Da
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
PTM: The N-terminus is blocked. & Contains a selenide-sulfide bond between Cys-497 and Sec-498. This bond is speculated to serve as redox active pair (By similarity).
SIMILARITY: SwissProt: Q16881 ## Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
MISCELLANEOUS: The active site is a redox-active disulfide bond.
SIZE: 499 amino acids; 54707 Da
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
PTM: The N-terminus is blocked. & Contains a selenide-sulfide bond between Cys-497 and Sec-498. This bond is speculated to serve as redox active pair (By similarity).
SIMILARITY: SwissProt: Q16881 ## Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
MISCELLANEOUS: The active site is a redox-active disulfide bond.
Qty/Pk:
100 µg
Brand Family:
Upstate
Format:
Affinity Purified
Host:
Rabbit
Product Name:
Anti-Thioredoxin Reductase 1 (TrxR1)
Antibody Type:
Polyclonal Antibody