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IB: 20μg of RIPA lysate from serum starved HEK293 cells was probed with anti-phospho-AMPKα (Thr172) (1:1000 dilution). Arrow indicates phospho-AMPKα ...
Anti-phospho-AMPKα (Thr172)
| 交差性 | 主要アプリケーション | 免疫動物 | フォーマット | 抗体タイプ |
|---|---|---|---|---|
| H, R | WB | Rabbit | Affinity Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: Q13131 # Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a cat
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Entrez Gene Number:
Entrez Gene Summary
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-cons
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Description:
Anti-phospho-AMPKα (Thr172)
Background Information:
AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. AMPK is an important regulator of glucose uptake, oxidation of fatty acids, cholesterol synthesis, lipogenesis, triglyceride synthesis, adipocyte lipolysis, glycolysis, and nitric oxide synthesis. AMPK is activated by exercise and more specifically, muscle contraction. During exercise, AMPK inhibits the enzyme acetyl-CoA carboxylase by phosphorylating the Ser79 site. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits and non-catalytic beta and gamma subunits. Alpha subunit has at least two isoforms (alpha 1 and alpha 2) which differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity.
Alternate Names:
- 5'-AMP-activated protein kinase, catalytic alpha-1 chain
- AMP -activate kinase alpha 1 subunit
- AMP-activated protein kinase, catalytic, alpha-1
- AMPK alpha 1
- AMPK alpha-1 chain
- protein kinase, AMP-activated, alpha 1 catalytic subunit
Specificity:
Recognizes phospho-AMPKα (Thr172), MW ~63 kDa. A non-specific protein was also detected, MW ~160 kDa.
Key Applications:
Western Blotting
Species Reactivity:
- Human
- Rat
Species Reactivity Note:
Human. Predicted to cross-react with rat based on sequence similarity.
Isotype:
IgG
Immunogen:
KLH-conjugated, synthetic peptide corresponding to amino acids surrounding phosphorylated Thr172 of human AMP-activated protein kinase alpha 2 (AMPKα2). The immunogenic site is conserved in AMPKα1, isoform 1 (Thr174), AMPKα1, isoform 2 (Thr189), and AMPKα2 (Thr172). Accession number NM_006251. The immunizing sequence is identical in rat.
Modifications:
Phosphorylation
Molecular Weight:
~63 kDa
Control:
Serum starved HEK293 cells
Presentation:
Purified rabbit polyclonal IgG in buffer containing 70% storage buffer 0.2 M Tris-glycine, pH 7.4, 0.15 M NaCl, 5 mg/mL BSA and 0.05% sodium azide and 30% glycerol.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Gene Symbol:
- PRKAA1
- MGC57364
- AMPK
- AMPK1
- MGC33776
- AMPKa1
Quality Assurance:
Evaluated by western blot in RIPA lysates from serum starved HEK293 cells.
Western Blot Analysis:
1:500-1:2000 dilutions of this antibody detected phospho-AMPKα (Thr172) in RIPA lysates from serum starved HEK293 cells.
Western Blot Analysis:
1:500-1:2000 dilutions of this antibody detected phospho-AMPKα (Thr172) in RIPA lysates from serum starved HEK293 cells.
Antibody Category:
Signaling
Antibody Sub-Category:
Insulin/Energy Signaling
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format Code:
APur
Concentration:
Variable
Antibody Type:
Polyclonal Antibody
Format:
Affinity Purified
Host:
Rabbit
Purification Method:
Immunoaffinity Purified