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WB: 20 micrograms of HeLa nuclear extract was probed with Anti-cdk2 (1μg/ml). Arrow indicates cdk2 (~33kDa).
Anti-cdk2
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IP, WB | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-cdk2
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes human p33cdk2 protein kinase.
Molecular Weight:
33 kDa
Immunogen:
peptide corresponding to amino acids 287-298 (C-QDVTKPVPHLRL) of human cdk2
Isotype:
IgG
Species Reactivity:
Human
Species Reactivity Note:
Predicted to cross-react with mouse, rat, non-human primates, and hamster based on sequence homology
Control:
Colon tissue
Quality Assurance:
routinely evaluated by immunoblot in nuclear extracts from HeLa cells
Purification Method:
Protein A purfied
Presentation:
Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CDK2
- p33(CDK2)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.
Key Applications:
- Immunoprecipitation
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P24941 # Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
SIZE: 298 amino acids; 33930 Da
SUBUNIT: Found in a complex with CABLES1, CCNA1 and CCNE1. Interacts with CABLES1 (By similarity). Interacts with UHRF2. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts with the Speedy/Ringo proteins SPDYA and SPDYC. Found in a complex with both SPDYA and CDKN1B/KIP1.
SIMILARITY: SwissProt: P24941 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
SIZE: 298 amino acids; 33930 Da
SUBUNIT: Found in a complex with CABLES1, CCNA1 and CCNE1. Interacts with CABLES1 (By similarity). Interacts with UHRF2. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts with the Speedy/Ringo proteins SPDYA and SPDYC. Found in a complex with both SPDYA and CDKN1B/KIP1.
SIMILARITY: SwissProt: P24941 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
Qty/Pk:
100 μg
Brand Family:
Upstate
Format:
Purified
Host:
Rabbit
Product Name:
Anti-cdk2
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody