Product Images
HeLa nuclear extract was probed with anti-Sp1 (2.0µg/ml). Arrows indicate Sp1 (~105 and 95kDa).
Western Blot Analysis:
HeLa lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-SP1 (1:500 dilution of this...
Anti-Sp1 Antibody
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | ChIP, EMSA, IF, IP, WB | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-Sp1 Antibody | 07-645
Promotional Text:
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Sp1, Mr 105 kDa and 95 kDa.
Molecular Weight:
Varies
Immunogen:
Full length human Sp1 protein.
Isotype:
IgG
Background Information:
Sp1 is a human transcription factor involved in gene expression in the early development of an organism. The protein is 785 amino acids long, with a molecular weight of 95 kDA. The SP1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Sp1 has always been described as a ubiquitous transcription factor that is required for the constitutive and inducible expression of a variety of genes, such as in cell cycle or mammalian development.
Species Reactivity:
- Human
- Mouse
- Rat
Species Reactivity Note:
Human and mouse. Expected to cross react with rat.
Application Notes:
Immunoprecipitation:
4 µg of this lot immunoprecipitated Sp1 from 500 µg of HeLa nuclear extract.
NOTE: A previous lot successfully immunoprecipitated the α-1 subunit using the Catch and Release v2.0 Reversible Immunoprecipitation System (Catalog # 17-500). See manual for more information and protocol: www.millipore.com
Chromatin Immunoprecipitation:
Reported by an independent laboratory.
Immunofluorescence:
Reported by an independent laboratory.
Electrophoretic Mobility Shift Assay (EMSA):
1 µL of a previous lot of this antibody has been reported by an independent laboratory to supershift DNA complexes containing recombinant Sp1 and endogenous Sp1 from HeLa cells in EMSA.
4 µg of this lot immunoprecipitated Sp1 from 500 µg of HeLa nuclear extract.
NOTE: A previous lot successfully immunoprecipitated the α-1 subunit using the Catch and Release v2.0 Reversible Immunoprecipitation System (Catalog # 17-500). See manual for more information and protocol: www.millipore.com
Chromatin Immunoprecipitation:
Reported by an independent laboratory.
Immunofluorescence:
Reported by an independent laboratory.
Electrophoretic Mobility Shift Assay (EMSA):
1 µL of a previous lot of this antibody has been reported by an independent laboratory to supershift DNA complexes containing recombinant Sp1 and endogenous Sp1 from HeLa cells in EMSA.
Control:
HeLa nuclear extract.
Quality Assurance:
Routinely evaluated by Western Blot on HeLa lysates.
Western Blot Analysis:
1:500 dilution of this lot detected SP1 on 10 μg of HeLa lysates.
Western Blot Analysis:
1:500 dilution of this lot detected SP1 on 10 μg of HeLa lysates.
Purification Method:
Protein A purfied
Presentation:
Purified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol.
Storage Conditions:
1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
TSFP1
Alternate Names:
- Sp1 transcription factor
- specificity protein 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Chromatin Immunoprecipitation (ChIP)
- Electrophoretic Mobility Shift Assay
- Immunofluorescence
- Immunoprecipitation
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P08047 # Binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. Can interact with G/C-rich motifs from serotonin receptor promoter.
SIZE: 785 amino acids; 80693 Da
SUBUNIT: Interacts with ATF7IP, ATF7IP2, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus IE62 protein and HIV-1 Vpr.
SUBCELLULAR LOCATION: Nucleus.
PTM: O-glycosylated; contains N-acetylglucosamine side chains.
SIMILARITY: SwissProt: P08047 ## Belongs to the Sp1 C2H2-type zinc-finger protein family. & Contains 3 C2H2-type zinc fingers.
SIZE: 785 amino acids; 80693 Da
SUBUNIT: Interacts with ATF7IP, ATF7IP2, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus IE62 protein and HIV-1 Vpr.
SUBCELLULAR LOCATION: Nucleus.
PTM: O-glycosylated; contains N-acetylglucosamine side chains.
SIMILARITY: SwissProt: P08047 ## Belongs to the Sp1 C2H2-type zinc-finger protein family. & Contains 3 C2H2-type zinc fingers.
Brand Family:
Upstate
Product Name:
Anti-Sp1 Antibody | 07-645
Concentration:
1.0 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µg
Format:
Purified
Host:
Rabbit