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3T3/A31 cell lysate was probed with anti-Cyclin E (1:500 dilution). Arrow indicates Cyclin E (~52kDa).
Anti-Cyclin E
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | IP, WB, IH | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Cyclin E
Trade Name:
Upstate (Millipore)
Specificity:
Cyclin E
Molecular Weight:
Mr 52kDa
Immunogen:
KLH-conjugated, synthetic peptide corresponding to amino acids 378-396 (GVLTPPHSSKKQSSEQETE) of rat Cyclin E
Isotype:
IgG
Background Information:
Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
Species Reactivity:
- Mouse
- Rat
Control:
3T3/A31 cell lysate, human colon carcinoma tissue
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from 3T3/A31 cells
Purification Method:
Immunoaffinity purified
Presentation:
PBS containing 0.1% sodium azide and 0.2% gelatin.
Storage Conditions:
stable for 1 year at 4°C from date of shipment
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CCNE1
- CCNE
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.
Key Applications:
- Immunoprecipitation
- Western Blotting
- Immunohistochemistry
UniProt Summary:
FUNCTION: SwissProt: P24864 # Essential for the control of the cell cycle at the G1/S (start) transition.
SIZE: 410 amino acids; 47077 Da
SUBUNIT: Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Interacts with retinoblastoma binding protein 3 and retinoblastoma-like protein 1. Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
PTM: Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: P24864 ## Belongs to the cyclin family. Cyclin E subfamily.
SIZE: 410 amino acids; 47077 Da
SUBUNIT: Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Interacts with retinoblastoma binding protein 3 and retinoblastoma-like protein 1. Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
PTM: Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: P24864 ## Belongs to the cyclin family. Cyclin E subfamily.
Qty/Pk:
100 µg
Brand Family:
Upstate
Format:
Affinity Purified
Host:
Rabbit
Product Name:
Anti-Cyclin E
Antibody Type:
Polyclonal Antibody