Anti-Rad51

Anti-Rad51

Upstate (Millipore)

RAD51

37kDa

GST fusion protein corresponding to full length human RAD51.

IgG

Human

Does not work in mouse.

Routinely evaluated by immunoblot.

Protein A purfied

2 years at -20°C from date of shipment

Q06609

• NM_002875.2
• NM_133487.1

• RAD51
• hRAD51
• RAD51L3
• HRAD51
• HsT16930
• BRCC5
• RECA
• HsRad51
• RAD51D
• HsRAD51
• RAD51A

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Transcript variants utilizing alternative polyA signals exist.

• Western Blotting
• Immunocytochemistry

FUNCTION: SwissProt: Q06609 # May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments.
SIZE: 339 amino acids; 36966 Da
SUBUNIT: Interacts with BRCA1, BRCA2 and either directly or indirectly with p53. Interacts with XRCC3, RAD54L and RAD54B. Part of a complex with RAD51C and RAD51B. Interacts with RAD51AP1. Interacts with CHEK1/CHK1, and this may require prior phosphorylation of CHEK1.
SUBCELLULAR LOCATION: Nucleus. Note=Colocalizes with RAD51AP1 to multiple nuclear foci upon induction of DNA damage.
TISSUE SPECIFICITY: Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
PTM: Phosphorylated. Phosphorylation of Thr-309 by CHEK1/CHK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination.DISEASE:SwissProt: Q06609 # Defects in RAD51 are associated with breast cancer (BC) [MIM:114480].
SIMILARITY: SwissProt: Q06609 ## Belongs to the recA family. RAD51 subfamily. & Contains 1 HhH domain.

200 µg

Upstate

Purified

Rabbit

Anti-Rad51

Polyclonal Antibody