Content Loading...
Content Loading...
Product Images
WB: Acid extracts from untreated (Lane 1) or sodium butyrate treated (Lane 2) HeLa cells were resolved by electrophoresis, transferred to nitrocellulo...
Anti-acetyl-Histone H3 (Lys79)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ELISA | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-acetyl-Histone H3 (Lys79)
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes acetyl-Histone H3 (Lys79), Mr 17 kDa. Does not recognize unmodified Histone H3.
Molecular Weight:
17 kDa
Epitope:
Acetylated Lys79 of Histone H3
Immunogen:
KLH-conjugated, synthetic peptide containing the sequence (…DF[acK]TD…) where [AcK] corresponds to acetyl-lysine 79 of human histone H3.
Modifications:
Acetylation
Isotype:
IgG
Background Information:
Methylation of Lysine 79 in Histone 3 acts as a marker of inactive chromatin regions. Methylation by Dot1 methylase on lysine-79 (Lys79) of Histone H3 is criticial for transcriptional silencing, and it is thought that silencing proteins such as Sir3 function by blocking Dot1 methylation. Likewise, it is predicted that acetylation of Lys79 may indicate active gene transcription.
Species Reactivity:
Human
Species Reactivity Note:
Broad species cross-reactivity expected due to sequence homology.
Application Notes:
Western Blot Analysis: A 1:1000-1:2000 dilution detected acetyl-Histone H3 (Lys79) in acid extracts from HeLa cells. Signal is increased in cells treated with sodium butyrate (Catalog # 19-137), a histone deacetylase inhibitor. No detection of recombinant, unmodified human Histone H3 (Catalog # 14-494) was observed.
Optimal working dilutions must be determined by the end user.
Optimal working dilutions must be determined by the end user.
Control:
Tested on acid extracted proteins from HeLa cells
Purification Method:
Immunoaffinity purified
Presentation:
Purified rabbit IgG in 70% storage buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide, 5 mg/mL BSA and 30% glycerol.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt. Avoid repeated freeze/thaw cycles.
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- H3F3A
- H3.3B
- H3F3
- MGC87783
- H3F3B
- H3.3A
- MGC87782
Alternate Names:
- H3K79Ac
- Histone H3 (acetyl K79)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
Key Applications:
- Western Blotting
- ELISA
Qty/Pk:
100 µL
Brand Family:
Upstate
Format:
Affinity Purified
Host:
Rabbit
Protein or Enzyme Type:
Histone
Product Name:
Anti-acetyl-Histone H3 (Lys79)
Antibody Type:
Polyclonal Antibody