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  • : 07-789
  • : 100 µL
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Immunoblot Analysis Lysates from untreated (lane 1) and Calyculin A treated (100nM for 30 min.) (lane 2) A431 cells were resolved by electroph...

Anti-phospho-AKT (Thr34) Antibody

Species Reactivity Key Applications Host Format Antibody Type
H WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-AKT (Thr34) Antibody
Promotional Text:
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes phospho-AKT (Thr34)
Molecular Weight:
60 kDa
Immunogen:
KLH conjugated peptide corresponding to amino acid region encompassing the human phospho-AKT (Thr34); Sequence highly conserved in mouse and rat.
Modifications:
Phosphorylation
Isotype:
Species Reactivity:
Human
Species Reactivity Note:
Predicted to cross react with Mouse and Rat due to high sequence homology, but have not been tested.
Quality Assurance:
Routinely evaluated by immunoblot.
Purification Method:
Immunoaffinity purified
Presentation:
100 μL of epitope specific affinity purified rabbit polyclonal IgG in 50% affinity storage buffer (PBS containing 1.0 mg/mL BSA and 0.05% sodium azide) and 50% glycerol.
Storage Conditions:
Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • AKT1
  • RAC
  • PRKBA
  • MGC99656
  • RAC-ALPHA
  • RAC-PK-alpha
  • C-AKT
  • PKB
  • AKT
Alternate Names:
Akt1, PKB, PRKBA, RAC, RAC-ALPHA
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Entrez Gene Summary:
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
UniProt Summary:
FUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
SIZE: 480 amino acids; 55686 Da
SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
TISSUE SPECIFICITY: In all human cell types so far analyzed.
DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
Brand Family:
Upstate
Product Name:
Anti-phospho-AKT (Thr34) Antibody
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µL
Format:
Affinity Purified
Host:
Rabbit
Applications:
Anti-phospho-AKT (Thr34) Antibody detects level of phospho-AKT (Thr34) & has been published & validated for use in WB.

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