Anti-phospho-PKA Catalytic β subunit (Ser338)

Anti-phospho-PKA Catalytic β subunit (Ser338)

Upstate (Millipore)

Recognizes phospho-PKA Catalytic β subunit (Ser338).

~40 kDa

Peptide corresponding to amino acid region encompassing the human, cow, and pig phospho-PKA Catalytic β (Ser338).

Phosphorylation

IgG

Mouse

Predicted to cross-react with human based on sequence homology (100% homologous).

Routinely evaluated by immunoblot.

Immunoaffinity purified

100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg²⁺ and Ca²⁺), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.

Stable for 2 years at -20°C from date of shipment.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

P22694

• NM_002731.2
• NM_182948.2
• NM_207578.1

• PRKACB
• PKACB
• MGC41879
• MGC9320
• DKFZp781I2452

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Three alternatively spliced transcript variants encoding distinct isoforms have been observed.

Western Blotting

FUNCTION: SwissProt: P22694 # Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.
COFACTOR: Magnesium.
SIZE: 351 amino acids; 40623 Da
SUBUNIT: A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note=Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm (By similarity).
TISSUE SPECIFICITY: Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoforms 3 and 4 are only epxressed in the brain.
PTM: Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively (By similarity).
SIMILARITY: SwissProt: P22694 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 protein kinase domain.

100 µL

Upstate

Affinity Purified

Rabbit

Anti-phospho-PKA Catalytic β subunit (Ser338)

Polyclonal Antibody