Ordering Information
- : 07-955
- : 100 μg
- : 1.0 mg/mL
Product Images
Western Blotting Analysis:
Representative lot data.
HeLa cell lysate was resolved by electrophoresis, transferred to PVDF membranes and pr...
Western Blotting (Snap ID) Analysis:
Representative lot data.
HeLa cell lysate was resolved by electrophoresis, transferred to PVDF membrane...
Anti-Hexim 1 Antibody
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Chp | WB, IP | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Hexim 1 Antibody | 07-955
Promotional Text:
Specificity:
This antibody recognizes Hexim1 at the N-terminus.
Molecular Weight:
53 kDa
Epitope:
N-terminus
Immunogen:
KLH-conjugated linear peptide corresponding to human Hexim 1 at and around the N-terminus.
Background Information:
Hexim1 is a transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. Hexim1 works in cooperation with 7SK snRNA to sequester P-TEFb in a large inactive 7SK snRNP complex, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. The 7SK snRNP complex is composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), Hexim1, Hexim2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Hexim1 also interacts with the N-CoR complex through NCOR1, ESR1, NR3C1, and may interact with NF-kappa-B through REL A. Hexim1 is located in the nucleus where it can bind with alpha-importin, and can also be found in the cytoplasm. Hexim1 is up-regulated by HMBA (hexamethylene bisacetamide) at the protein level and down-regulated by estrogen.
Species Reactivity:
- Human
- Chimpanzee
Species Reactivity Note:
Demonstrated to react with human. Predicted to react with chimpanzee and Rhesus monkey based on 100% sequence homology.
Application Notes:
Western Blot (SNAP ID) Analysis: 4 µg/ml from a previous lot detected Hexim 1 on 10 µg of HeLa cell lysate.
Immunoprecipitation Analysis: A representative lot of this antibody was used by an independent laboratory for IP.
Immunoprecipitation Analysis: A representative lot of this antibody was used by an independent laboratory for IP.
Control:
HeLa cell lysate
Quality Assurance:
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.1 µg/ml of this antibody detected Hexim 1 on 10 µg of HeLa cell lysate.
Western Blot Analysis: 0.1 µg/ml of this antibody detected Hexim 1 on 10 µg of HeLa cell lysate.
Purification Method:
Affinity Purfied
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CLP-1
- CLP1
- EDG1
- HIS1
- HMBA-inducible
- MAQ1
- Hexim1
Alternate Names:
- Cardiac lineage protein 1
- Estrogen down-regulated gene 1 protein
- Hexamethylene bis-acetamide-inducible protein 1
- Menage a quatre protein 1
- hexamethylene bis-acetamide inducible 1
- hexamethylene bisacetamide-inducible protein
- hexamethylene-bis-acetamide-inducible transcript 1
- hexamthylene bis-acetamide inducible 1
- menage a quatre 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Immunoprecipitation
Entrez Gene Summary:
Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns.
UniProt Summary:
FUNCTION: Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity.
SUBUNIT STRUCTURE: Homo-oligomer and hetero-oligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Binds alpha-importin and is mostly nuclear.
TISSUE SPECIFICITY: Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues.
INDUCTION: Up-regulated by HMBA (hexamethylene bisacetamide) (at protein level). Down-regulated by estrogen.
DOMAIN: The coiled-coil domain mediates oligomerization.
MISCELLANEOUS: Inhibits Tat activity which is required for HIV-1 transcription.
SEQUENCE SIMILARITIES: Belongs to the HEXIM family.
SUBUNIT STRUCTURE: Homo-oligomer and hetero-oligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Binds alpha-importin and is mostly nuclear.
TISSUE SPECIFICITY: Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues.
INDUCTION: Up-regulated by HMBA (hexamethylene bisacetamide) (at protein level). Down-regulated by estrogen.
DOMAIN: The coiled-coil domain mediates oligomerization.
MISCELLANEOUS: Inhibits Tat activity which is required for HIV-1 transcription.
SEQUENCE SIMILARITIES: Belongs to the HEXIM family.
Product Name:
Anti-Hexim 1 Antibody (07-955)
Concentration:
1.0 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit