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Cat. No. 08-126; SDS-PAGE and Coomassie Stain:
Purity was assessed by SDS-PAGE and Coomassie blue staining using 5 µg of recombinant human Vitr...
Vitronectin, human recombinant
Recommended Replacement for: CC080Description:
Vitronectin, human recombinant
Trade Name:
Upstate (Millipore)
Qty/Pk:
500 µg
Key Applications:
- Positive Control
- Cell Culture
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.
UniProt Summary:
FUNCTION: SwissProt: P04004 # Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
SIZE: 478 amino acids; 54306 Da
SUBUNIT: Exists in two forms: a single chain 75 kDa form (V75) and a clipped form composed of two chains (65 kDa and 10 kDa) (V65+V10) which are held together by a disulfide bond. Interacts with SERPINE1/PAI1 and insulin.
SUBCELLULAR LOCATION: Secreted, extracellular space.
TISSUE SPECIFICITY: Plasma.
DOMAIN: SwissProt: P04004 The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
PTM: Sulfated on 2 tyrosine residues. & N- and O-glycosylated (By similarity). & Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading. & It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.SIMILARITY: Contains 4 hemopexin-like domains. & Contains 1 SMB (somatomedin-B) domain.
SIZE: 478 amino acids; 54306 Da
SUBUNIT: Exists in two forms: a single chain 75 kDa form (V75) and a clipped form composed of two chains (65 kDa and 10 kDa) (V65+V10) which are held together by a disulfide bond. Interacts with SERPINE1/PAI1 and insulin.
SUBCELLULAR LOCATION: Secreted, extracellular space.
TISSUE SPECIFICITY: Plasma.
DOMAIN: SwissProt: P04004 The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
PTM: Sulfated on 2 tyrosine residues. & N- and O-glycosylated (By similarity). & Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading. & It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.SIMILARITY: Contains 4 hemopexin-like domains. & Contains 1 SMB (somatomedin-B) domain.
Species:
Human
Molecular Weight:
78 kDa
Quality Assurance:
Routinely evaluated by cell adhesion assay.
Stem Cell Type:
- Human Embryonic Stem Cells
- Mesenchymal Stem Cells
- Neural Stem Cells
- Hematopoietic Stem Cells
- Epithelial Cells
- Pancreatic Stem Cells
- Induced Pluripotent Stem Cells
Stem Cell Workflow Stage:
- Maintenance & Expansion
- General Cell Culture
Brand Family:
Upstate
Source:
N-terminal, GST-tagged, full-length human Vitronectin expressed in E. coli
Presentation:
Liquid in 10mM Sodium Phosphate, pH 7.1, 150mM Sodium Chloride, 0.1mM DTT, 0.1mM PMSF and 50% glycerol.
UniProt Number:
Storage Conditions:
6 months at -20°C from date of shipment
Cell Culture Reagent Type:
- Stem Cell Reagents
- Attachment Factors
Replaces:
CC080
Replaced By:
CC080
Gene Symbol:
- VTN
- VN
- epibolin
- V75
- VNT
- S-protein
Protein or Enzyme Type:
- Extracellular Matrix Proteins
- Recombinant Protein
Product Name:
Vitronectin, human recombinant
Entrez Gene Number:
Concentration:
1.5 mg/mL