Close
Immunocytochemistry
PI3-Kinase, p110β staining of human kidney 2 mm array spot. Tissue pre-treated with Citrate pH 6.0 antigen-retrieval. Anti-PI3 Kinase, p110β diluted to 1:100, IHC-Select Detection with HRP-DAB. Immunoreactivity is see as a staining to include the thin and distal microtubules. Additional data shown on page 2.
Confocal Immunocytochemistry Analysis
Hela cells were fixed, permeablized, and stained with anti- PI3K, p110β (Cy3, red), DAPI (blue, nuclei), and Phalloidin-AlexaFluor®488 (actin, green). Figure on the left has the DAPI filter off.
Immunocytochemistry: PI3-Kinase, p110b staining in colorectal carcinoma. Tissue pre-treated with Citrate pH 6.0 antigen-retrieval. Anti-PI3 Kinase, p110b diluted to 1:100, IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing as a plasma membrane staining pattern.
Immunocytochemistry
PI3-Kinase, p110β staining of human skeletal muscle. Tissue pre-treated with Citrate pH 6.0 antigen-retrieval. Anti-PI3 Kinase, p110β diluted to 1:100, IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing as a staining pattern of cross banding striated muscle fibers.
Immunoprecipitation
10 μg of Anti-PI3 kinase, p110β was used to immunoprecipitate from 500 µg of Jurkat whole cell lysate. The antibody was collected on Protein A beads and eluted with sample buffer. 5 µL of Jurkat whole cell lysate was then resolved via SDS-PAGE, transferred to PVDF and probed with 0.5 µg of rabbit polyclonal Anti-PI3 kinase, p110β (Cat. No. 09-482). Proteins were visualized using anti-rabbit-HRP conjugate and a Vizualizer™ Spray&Glow™ ECL system (Cat. No. 17-373).
Western Blot Analysis:
Representative lot data.
Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110β (0.05 µg/mL).
Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection.
Arrow indicates PI3 Kinase, p110β (~110 kDa).