Ordering Information
- : 09-722
- : 100 μg
- : 1 mg/mL
Product Images
Western Blot (Snap ID) Analysis:
Representative lot data.
LNCAP cell lysate was resolved by electrophoresis, transferred to PVDF membranes...
Western Blot Analysis:
Representative lot data.
LNCAP lysate was resolved by electrophoresis, transferred to PVDF membranes and probed wit...
Anti-XBP1 Antibody
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-XBP1 Antibody | 09-722
Promotional Text:
Specificity:
This antibody recognizes XBP1 at the C-terminus.
Molecular Weight:
29 kDa
Epitope:
N-terminus
Immunogen:
KLH-conjugated linear peptide corresponding to human XBP1 at and around the N-terminus.
Background Information:
X-box Protein 1 (XBP1) is a transcription factor that belongs to the bZIP family. XBP1 plays an important role in the functioning of the immune system and in the cellular stress response. It is essential for the differentiation of plasma cells and is involved in the regulation of the MHC class II genes. XBP1 becomes initiated during an endoplasmic reticulum (ER) stress response by binding to the unfolded protein response element (UPRE) which activates the UPR target genes. Mutations in XBP1 have been associated with mental disorders.
Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with human. Peptide homology with rat and mouse is 86%.
Application Notes:
Western Blot (SNAP ID) Analysis: 6 µg/ml from a representative lot detected XBP1 on 10 µg of LNCAP cell lysate.
Control:
LNCAP cell lysate
Quality Assurance:
Evaluated by Western Blot in LNCAP cell lysate.
Western Blot Analysis: 2 µg/ml of this antibody detected XBP1 on 10 µg of LNCAP cell lysate.
Western Blot Analysis: 2 µg/ml of this antibody detected XBP1 on 10 µg of LNCAP cell lysate.
Purification Method:
Affinity Purfied
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- XBP1
- TREB5
- XBP-1
- XBP2
Alternate Names:
- Tax-responsive element-binding protein 5
- X-box binding protein 1
- X-box-binding protein-1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Entrez Gene Summary:
This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.
UniProt Summary:
FUNCTION: Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N3[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.
SUBCELLULAR LOCATION: Nucleus.
INDUCTION: Up-regulated by ATF6 via direct binding to the ERSE in response to endoplasmic reticulum stress.
INVOLVEMENT IN DISEASE: Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature.
SEQUENCE SIMILARITIES: Belongs to the bZIP family.
Contains 1 bZIP domain
SUBCELLULAR LOCATION: Nucleus.
INDUCTION: Up-regulated by ATF6 via direct binding to the ERSE in response to endoplasmic reticulum stress.
INVOLVEMENT IN DISEASE: Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature.
SEQUENCE SIMILARITIES: Belongs to the bZIP family.
Contains 1 bZIP domain
Product Name:
Anti-XBP1
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit