Ordering Information
- : 09-723
- : 100 µg
- : 1 mg/mL
Product Images
Western Blot Analysis:
Representative lot data.
NIH/3T3 cell lysate was resolved by electrophoresis, transferred to PVDF and probed with Ant...
Western Blot Analysis:
Representative lot data.
Human brain lysate was resolved by electrophoresis, transferred to PVDF and probed with Anti...
 (0.5 µg/mL). <br />Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. <br /><br />Arrow indicates RNF2 (Ring1b) <br />(~45 kDa)<br />'))
 (1 µg/mL from a previous lot). <br />Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. <br /><br />Arrow indicates RNF2 (Ring1b) (~45 kDa)<br />'))
 Analysis:<br />Representative lot data.<br />NIH3T3 lysate was resolved by electrophoresis, transferred to PVDF and probed with Anti-RNF2 (Ring1b) (1.5 µg/mL from a previous lot). <br />Proteins were visualized using a Snap i.d., a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. <br /><br />Arrow indicates RNF2 (Ring1b) (~45 kDa)<br />'))
Anti-RNF2 (Ring1b) Antibody
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, B, Ch, Ca | WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-RNF2 (Ring1b) Antibody | 09-723
Promotional Text:
Specificity:
This antibody recognizes RNF2 (Ring1b).
Molecular Weight:
~45 kDa
Immunogen:
KLH-conjugated linear peptide corresponding to human RNF2 (Ring1b).
Background Information:
RNF2 (ring finger 2), also known as Ring1b, is one of the polycomb groups of proteins, which are important for proper development and stem cell renewal. These proteins form complexes that are essential for the transcriptional repression of genes involved in cell proliferation. This is done through post-translational modification and binding to histones. RNF2 is a critical component of that process. Changes in the expression of these proteins have been linked to cancer. RNF2 has been shown to interact with huntingtin interacting protein 2 (HIP2), a ubiquitin-conjugating enzyme. Studies have also shown that RNF2 is able to suppress the activity of transcription factor CP2.
Species Reactivity:
- Human
- Mouse
- Rat
- Bovine
- Chicken
- Canine
Species Reactivity Note:
Demonstrated to react with human, mouse, and rat. Predicted to react with chicken, canine (dog), and bovine (cow) based on 100% sequence homology.
Application Notes:
Western Blot Analysis: 1 µg/mL from a previous lot detected RNF2 (Ring1b) in 10 µg of human brain tissue lysate.
Western Blot (SNAP ID) Analysis:
1.5 µg/mL from a previous lot detected RNF2 (Ring1b) in 10 µg of NIH/3T3 cell lysate.
Western Blot (SNAP ID) Analysis:
1.5 µg/mL from a previous lot detected RNF2 (Ring1b) in 10 µg of NIH/3T3 cell lysate.
Control:
NIH/3T3 cell lysate
Quality Assurance:
Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: 0.5-1 µg/mL of this antibody detected RNF2 (Ring1b) in 10 µg of NIH/3T3 cell lysate.
Western Blot Analysis: 0.5-1 µg/mL of this antibody detected RNF2 (Ring1b) in 10 µg of NIH/3T3 cell lysate.
Purification Method:
Affinity Purfied
Presentation:
Purified rabbit polyclonal containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.
Storage Conditions:
Stable at 2-8°C for 1 year from date of recepit.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- BAP-1
- BAP1
- DING
- EC 6.3.2.-
- HIPI3
- OTTHUMP00000033405
- OTTHUMP00000060668
- RING1B
- RING1b
- RING2
Alternate Names:
- DinG protein
- HIP2-interacting protein 3
- Huntingtin-interacting protein 2-interacting protein 3
- RING finger protein 1B
- RING finger protein BAP-1
- ring finger protein 2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Entrez Gene Summary:
Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity.
UniProt Summary:
FUNCTION: E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 and BMI1/PCGF4 may rather act as a modulator of RNF2/RING2 activity.
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT STRUCTURE: Component of some chromatin-associated Polycomb complex (PcG). Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2/RING2, MBLR, L3MBTL2 and YAF2. Component of chromatin-associated class II PcG repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110, BMI1/PCGF4, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 and RNF2/RING2. Interacts with RYBP, HIP2 and TFCP2. Association to the chromosomal DNA is cell-cycle dependent. Component of repressive BCOR complex containing Polycomb group subcomplex at least composed of RYBP, PCGF1, BCOR and RING1. SUBCELLULAR LOCATION: Nucleus By similarity. Note: Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells. The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells By similarity.
SEQUENCE SIMILARITIES: Contains 1 RING-type zinc finger.
SEQUENCE CAUTIION: The sequence CAI17849.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAI17850.1 differs from that shown. Reason: Erroneous gene model prediction.
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT STRUCTURE: Component of some chromatin-associated Polycomb complex (PcG). Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2/RING2, MBLR, L3MBTL2 and YAF2. Component of chromatin-associated class II PcG repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110, BMI1/PCGF4, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 and RNF2/RING2. Interacts with RYBP, HIP2 and TFCP2. Association to the chromosomal DNA is cell-cycle dependent. Component of repressive BCOR complex containing Polycomb group subcomplex at least composed of RYBP, PCGF1, BCOR and RING1. SUBCELLULAR LOCATION: Nucleus By similarity. Note: Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells. The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells By similarity.
SEQUENCE SIMILARITIES: Contains 1 RING-type zinc finger.
SEQUENCE CAUTIION: The sequence CAI17849.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAI17850.1 differs from that shown. Reason: Erroneous gene model prediction.
Product Name:
Anti-RNF2 (Ring1b)
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µg
Format:
Affinity Purified
Host:
Rabbit