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C-Jun DNA Binding Activity in PMA Treated HeLa Nuclear Extract
PMA Treated HeLa Nuclear Extract
Description:
PMA Treated HeLa Nuclear Extract
Trade Name:
Upstate (Millipore)
Qty/Pk:
250 µg
Product Overview:
Biologically active nuclear extract preparation. Cells were treated with 50 ng/mL phorbol 12-myristate 13-acetate for 2 hours at 37oC. Nuclei were isolated and then lysed in nuclear extraction buffer (20 mM HEPES, pH 7.9, 1.5 mM MgCl2, 0.42 M NaCl, 0.2 mM EDTA, 1% IGEPAL CA-630, 25% glycerol, and protease inhibitor cocktail). Protein concentration was determined and then normalized to 2.5 mg/mL.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Lysate Type:
Cell Lysate
Species:
Human
Quality Assurance:
Routinely tested by Transcription Factor Assay.
Brand Family:
Upstate
Source:
Human HeLa cells.
Presentation:
4-25 µL frozen aliquots are provided at a concentration of 2.5mg/ml.
Storage Conditions:
Stable for 6 months at -80°C from date of shipment.
Product Name:
PMA Treated HeLa Nuclear Extract