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Casein Kinase 2, active
Description:
Casein Kinase 2, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
10 µg
Product Overview:
Recombinant full-length human Casein Kinase 2 protein, containing an &alpyha;-subunit N-terminal 6His tag and a β-subunit N-terminal GST tag.
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene.
UniProt Summary:
FUNCTION: SwissProt: P68400 # Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.
SIZE: 391 amino acids; 45144 Da
SUBUNIT: Tetramer composed of an alpha chain, an alpha' and two beta chains. Also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation. Interacts with RNPS1.
SIMILARITY: SwissProt: P68400 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily. & Contains 1 protein kinase domain.
SIZE: 391 amino acids; 45144 Da
SUBUNIT: Tetramer composed of an alpha chain, an alpha' and two beta chains. Also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation. Interacts with RNPS1.
SIMILARITY: SwissProt: P68400 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily. & Contains 1 protein kinase domain.
Species:
Human
Molecular Weight:
heterotetramer of 49kDa α-subunit and 53kDa β-subunit (α2β2)
Quality Assurance:
Routinely evaluated by phosphorylation of the Casein Kinase 2 Substrate Peptide.
Brand Family:
Upstate
Source:
Expressed in Sf21 insect cells.
Presentation:
20mM MOPS, pH 7.4, 1mM EDTA, 0.1% (v/v) Triton X-100, 0.3% 2-mercaptoethanol, 1mM benzamidine, 50% glycerol.
Protein Target:
CK2
UniProt Number:
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Storage Conditions:
1 year at -20°C.
Target Sub-Family:
CMGC
Promotional Text:
This enzyme is part of KinaseProfiler™, a custom inhibitor selectivity discovery service.
Packaging:
2 vials at 5μg each
Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-197M when ordering the 250μg size.
Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-197M when ordering the 250μg size.
Gene Symbol:
- CSNK2A1
- CK2A1
- CKII
Protein or Enzyme Type:
- Kinases
- Signal Transduction
Product Name:
Casein Kinase 2, active
Entrez Gene Number:
Purification Method:
Sequential chromatography using heparin Sepharose™ and Mono Q anion exchange resin