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SDS-PAGE: 2.5 micrograms of AMP-Activated Protein Kinase, active was assessed by SDS-PAGE and Coomassie blue staining.
AMPK, active
Description:
AMPK, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
5 units
Product Overview:
Holoenzyme comprised of 3 subunits.
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
UniProt Summary:
FUNCTION: SwissProt: Q13131 # Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
COFACTOR: Magnesium.
SIZE: 550 amino acids; 62808 Da
SUBUNIT: Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits. Interacts with FNIP1.
SIMILARITY: SwissProt: Q13131 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. & Contains 1 protein kinase domain.
COFACTOR: Magnesium.
SIZE: 550 amino acids; 62808 Da
SUBUNIT: Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits. Interacts with FNIP1.
SIMILARITY: SwissProt: Q13131 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. & Contains 1 protein kinase domain.
Species:
Rat
Quality Assurance:
Routinely evaluated by phosphorylation of SAMS substrate peptide
Brand Family:
Upstate
Source:
Partially purified from rat liver.
Protein Target:
AMPK
UniProt Number:
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Storage Conditions:
1 year at -70°C
Target Sub-Family:
CAMK
Promotional Text:
This enzyme is part of KinaseProfiler™, a custom inhibitor selectivity discovery service.
Gene Symbol:
- PRKAA1
- MGC57364
- AMPK
- MGC33776
- AMPKa1
- AMPK1
Protein or Enzyme Type:
- Kinases
- Signal Transduction
Product Name:
AMPK, active
Entrez Gene Number:
Purification Method:
2.5%-6.0% PEG, DEAE Sepharose™, Blue Sepharose™, 50% Ammonium Sulfate, Q-Sepharose™ and Sephacryl™ S200 chromatography.