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  • : 14-314
  • : 10 µg
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Sample enzyme activity data. See certificate of analysis for lot specific activity data.

Syk Protein, active, 10 μg

Description:
Syk, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
10 µg
Product Overview:
Recombinant full-length human Syk, containing an N-terminal His6-tag
Applications:
Active, recombinant full-length human Syk, containing an N-terminal His6-tag, for use in Kinase Assays.
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UniProt Summary:
FUNCTION: SwissProt: P43405 # Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion (By similarity).
SIZE: 635 amino acids; 72066 Da
SUBUNIT: Interacts with CBL and SLA when it is phosphorylated. The interaction with SLA may link it to CBL, leading to its destruction. Interacts with phosphorylated NFAM1 (By similarity). Interacts with Epstein-Barr virus LMP2A. Interacts through its SH2 domains with the phosphorylated ITAM domain of CD79A which stimulates SYK autophosphorylation and activation.
PTM: Autophosphorylated. & Phosphorylation on Tyr-323 creates a binding site for c-Cbl, an adapter protein that serves as a negative regulator of BCR- stimulated calcium ion signaling (By similarity). & Phosphorylation on Tyr-348 and Tyr-352 enhances the phosphorylation and activation of phospholipase C-gamma and the early phase of calcium ion mobilization via a phosphoinositide 3- kinase-independent pathway (By similarity). & Ubiquitinated by CBLB after BCR activation; which promotes proteasomal degradation (By similarity).
SIMILARITY: SwissProt: P43405 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily. & Contains 1 protein kinase domain. & Contains 2 SH2 domains.
Species:
Human
Molecular Weight:
73kDa
Brand Family:
Upstate
Source:
Expressed by baculovirus in Sf21 insect cells
Protein Target:
Storage Conditions:
1 year at -70C
Target Sub-Family:
Promotional Text:
This enzyme is part of KinaseProfiler™, a custom inhibitor selectivity discovery service.
Gene Symbol:
Product Name:
Syk, active
Quality Assurance:
routinely evaluated by phosphorylation of Poly (Glu4-Tyr)
UniProt Number:
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Packaging:
Also available in 250μg size -- call for pricing and availability and reference catalog number 14-314M when ordering the 250μg size.
Entrez Gene Number:
Purification Method:
Ni2+/NTA-agarose

Product Resources

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