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SDS-PAGE and Coomassie Stain: Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 µg of Raf-1 (truncated), active. Arrow indicates Ra...
Raf-1 (truncated), active
Description:
Raf-1 (truncated), active
Trade Name:
Upstate (Millipore)
Qty/Pk:
10 µg
Product Overview:
N-terminal GST-tagged recombinant human Raf-1 residues 306-end, containing the mutations Y340D and Y341D
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. [Contributed text]
UniProt Summary:
FUNCTION: SwissProt: P04049 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
COFACTOR: Binds 2 zinc ions per subunit.
SIZE: 648 amino acids; 73052 Da
SUBUNIT: Interacts with Ras proteins. The interaction with Ras is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3, which inhibits its pro-apoptotic activity.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY:SwissProt: P04049 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
COFACTOR: Binds 2 zinc ions per subunit.
SIZE: 648 amino acids; 73052 Da
SUBUNIT: Interacts with Ras proteins. The interaction with Ras is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3, which inhibits its pro-apoptotic activity.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY:SwissProt: P04049 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
Species:
Human
Molecular Weight:
65kDa
Quality Assurance:
routinely evaluated by phosphorylation of MBP (Catalog # 13-104) substrate in vitro
Brand Family:
Upstate
Source:
Expressed by baculovirus in Sf21 insect cells
Protein Target:
c-RAF
UniProt Number:
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Storage Conditions:
1 year at -70°C
Target Sub-Family:
TKL
Promotional Text:
This enzyme is part of KinaseProfiler™, a custom inhibitor selectivity discovery service.
Packaging:
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-352M when ordering the 250 µg size.
Gene Symbol:
- RAF1
- C-RAF
- cRaf
- Raf-1
- c-Raf
- CRAF
- RAF
Protein or Enzyme Type:
- Kinases
- Signal Transduction
Product Name:
Raf-1 (truncated), active
Entrez Gene Number:
Purification Method:
Glutathione agarose affinity chromatography