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Sample enzyme activity data. See certificate of analysis for lot specific activity data.
Cdk1/cyclin B, active
Description:
Cdk1/cyclin B, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
10 µg
Product Overview:
C-terminal His6-tagged human full length cdk1, and N-terminal GST-tagged human full length cyclin B
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.
UniProt Summary:
FUNCTION: SwissProt: P06493 # Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
SIZE: 297 amino acids; 34095 Da
SUBUNIT: Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with DLG7. Isoform 2 is unable to complex with cyclin B1 and also fails to bind to the CDK inhibitor p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase.
SUBCELLULAR LOCATION: Nucleus (By similarity).
SIMILARITY: SwissProt: P06493 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
SIZE: 297 amino acids; 34095 Da
SUBUNIT: Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with DLG7. Isoform 2 is unable to complex with cyclin B1 and also fails to bind to the CDK inhibitor p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase.
SUBCELLULAR LOCATION: Nucleus (By similarity).
SIMILARITY: SwissProt: P06493 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
Species:
Human
Molecular Weight:
35kDa (cdk1) and 75kDa (cyclin B)
Quality Assurance:
routinely evaluated by phosphorylation of the Histone H1 substrate
Brand Family:
Upstate
Source:
expressed individually by baculovirus in Sf21 insect cells
Protein Target:
CDK1/cyclinB
UniProt Number:
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Storage Conditions:
6 months at -70°C
Target Sub-Family:
CMGC
Promotional Text:
This enzyme is part of KinaseProfiler™, a custom inhibitor selectivity discovery service.
Packaging:
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-450M when ordering the 250 µg size.
Protein/Isoform Description:
Cdk1/cdc2 is a Ser/Thr kinase that serves as the catalytic subunit of the M-phase Promoting Factor (MPF) complex. MPF is critical for appropriate regulation of cell cycle progression, specifically for control of the G1/S and G2/M phase transition. Cdc2 activity is controlled by the presence of Cyclins, which appear and are then degraded at specific times during the cell cycle in a tightly regulated manner. cdc2 activity is also regulated by phosphorylation.
Gene Symbol:
- CDC2
- CDK1
- MGC111195
- DKFZp686L20222
- CDC28A
Protein or Enzyme Type:
- Kinases
- Signal Transduction
Product Name:
Cdk1/cyclin B, active
Entrez Gene Number:
Purification Method:
Purified using Ni2+/NTA-agarose and GST-agarose, respectively. The cdk1 is then activated using CAK and repurified by Q Sepharose™ and Ni2+/NTA-agarose