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Sample enzyme activity data. See certificate of analysis for lot specific activity data.
Tie2, active
Description:
Tie2, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
10 µg
Product Overview:
N-terminal His6-tagged recombinant human Tie2 residues 771-end
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.
UniProt Summary:
FUNCTION: SwissProt: Q02763 # This protein is a protein tyrosine-kinase transmembrane receptor for angiopoietin 1. It may constitute the earliest mammalian endothelial cell lineage marker. Probably regulates endothelial cell proliferation, differentiation and guides the proper patterning of endothelial cells during blood vessel formation.
SIZE: 1124 amino acids; 125811 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.
DISEASE:SwissProt: Q02763 # Defects in TEK are a cause of dominantly inherited venous malformations (VMCM) [MIM:600195]; an error of vascular morphogenesis characterized by dilated, serpiginous channels.
SIMILARITY: SwissProt: Q02763 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily. & Contains 3 EGF-like domains. & Contains 3 fibronectin type-III domains. & Contains 2 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
SIZE: 1124 amino acids; 125811 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.
DISEASE:SwissProt: Q02763 # Defects in TEK are a cause of dominantly inherited venous malformations (VMCM) [MIM:600195]; an error of vascular morphogenesis characterized by dilated, serpiginous channels.
SIMILARITY: SwissProt: Q02763 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily. & Contains 3 EGF-like domains. & Contains 3 fibronectin type-III domains. & Contains 2 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
Species:
Human
Molecular Weight:
42kDa
Quality Assurance:
routinely evaluated by phosphorylation of Poly (Glu4-Tyr) (4:1)
Brand Family:
Upstate
Source:
expressed by baculovirus in Sf21 insect cells
Protein Target:
Tie2
UniProt Number:
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Target Sub-Family:
TK
Storage Conditions:
6 months at -70C
Promotional Text:
This enzyme is part of KinaseProfiler™, a custom inhibitor selectivity discovery service.
Packaging:
2 vials at 5μg each
Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-540M when ordering the 250μg size.
Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-540M when ordering the 250μg size.
Gene Symbol:
- TEK
- CD202B
- TIE2
- VMCM1
- VMCM
- hTIE2
- CD202b
- TIE-2
Protein or Enzyme Type:
- Kinases
- Signal Transduction
Product Name:
Tie2, active
Entrez Gene Number:
Purification Method:
Ni2+/NTA-agarose