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Alk, active
Description:
Alk, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
10 µg
Product Overview:
N-terminal His6-tagged, recombinant human ALK, residues 1058-end
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The 2;5 chromosomal translocation is frequently associated with anaplastic large cell lymphomas (ALCLs). The translocation creates a fusion gene consisting of the ALK (anaplastic lymphoma kinase) gene and the nucleophosmin (NPM) gene: the 3' half of ALK, derived from chromosome 2, is fused to the 5' portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK (leukocyte tyrosine kinase). ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system.
UniProt Summary:
FUNCTION: SwissProt: Q9UM73 # Orphan receptor with a tyrosine-protein kinase activity. Appears to play an important role in the normal development and function of the nervous system. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif.
SIZE: 1620 amino acids; 176428 Da
SUBUNIT: Homodimer. When bound to ligand.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.
PTM: N-glycosylated.
DISEASE: SwissProt: Q9UM73 # A chromosomal aberration involving ALK is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with NPM1. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated. The constitutively active fusion proteins are responsible for 5-10% of non-Hodgkin lymphomas. & A chromosomal aberration involving ALK is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with CARS; translocation t(2;4)(p23;q21) with SEC31A. & A chromosomal aberration involving ALK is associated with anaplastic large-cell lymphoma (ALCL). Translocation t(2;17)(p23;q25) with ALO17.
SIMILARITY: SwissProt: Q9UM73 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. & Contains 1 LDL-receptor class A domain. & Contains 2 MAM domains. & Contains 1 protein kinase domain.
SIZE: 1620 amino acids; 176428 Da
SUBUNIT: Homodimer. When bound to ligand.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.
PTM: N-glycosylated.
DISEASE: SwissProt: Q9UM73 # A chromosomal aberration involving ALK is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with NPM1. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated. The constitutively active fusion proteins are responsible for 5-10% of non-Hodgkin lymphomas. & A chromosomal aberration involving ALK is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with CARS; translocation t(2;4)(p23;q21) with SEC31A. & A chromosomal aberration involving ALK is associated with anaplastic large-cell lymphoma (ALCL). Translocation t(2;17)(p23;q25) with ALO17.
SIMILARITY: SwissProt: Q9UM73 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. & Contains 1 LDL-receptor class A domain. & Contains 2 MAM domains. & Contains 1 protein kinase domain.
Species:
Human
Molecular Weight:
63.8kDa
Quality Assurance:
routinely evaluated by phosphorylation of IGF-1Rtide
Brand Family:
Upstate
Source:
expressed by baculovirus in Sf21 cells
Protein Target:
ALK
UniProt Number:
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Storage Conditions:
6 months at -70°C from date of shipment
Target Sub-Family:
TK
Protein/Isoform Description:
Anaplastic Lymphoma Kinase (ALK) is a receptor Tyr kinase implicated in anaplastic large cell lymphoma. The ALK gene may undergo a chromosomal translocation resulting in fusion with the gene for Nucleophosmin; the resulting protein in transforming. ALK is thought to be normally involved in neuronal development. ALK4 (ACVR1B activin A receptor, type IB; ACVRLK4) is a transmembrane type IB receptor serine kinase for activin or activin-like ligands. ALK7 (ACVR1C activin A receptor, type IC; ACVRLK4) is a transmembrane type IC receptor serine kinase for activin or activin-like ligands. Activins, are dimeric factors that belong to the TGFβ-superfamily of signaling proteins. Activins signal through the hetrodimeric complex that includes two type I (I and IB) and two type II (II and IIB) receptors. The type I receptors (i.e. ALK4 and ALK7) contain a Ser/Thr kinase domain in its cytoplasmic domain that is essential for signaling, while the type II receptors are essential for ligand binding (Harrison, 2004 & Gray, 2000). Receptor activation involves ligand binding to the promiscuous type II receptor that is known to bind to many ligands. This leads to the recruitment of ALK4 and ALK7 and the formation of a stable heterodimer that results in the phosphorylation and activation of ALK4 and/or ALK7 (Harrison, 2004 & Gray, 2000).
Gene Symbol:
- ALK
- CD246
- TFG/ALK
Protein or Enzyme Type:
Kinases
Product Name:
Alk, active
Entrez Gene Number:
Purification Method:
Ni2+/NTA-agarose