Ordering Information
- : 14-602
- : 20 µg
PI3 Kinase (p110α/p85α) Protein, active, 20 μg
Description:
PI3 Kinase (p110α/p85α), active
Trade Name:
Upstate (Millipore)
Qty/Pk:
20 µg
Product Overview:
Complex of N-terminal His6-tagged recombinant full-length human p110α and untagged, recombinant, full length, human p85α
Applications:
20 µg of PI3 Kinase (p110α/p85α). Active complex of N-terminal His6-tagged recombinant full-length human p110α and untagged, recombinant, full length, human p85α. For use in Kinase Assays.
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
UniProt Summary:
FUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
SIZE: 1068 amino acids; 124284 Da
SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).
DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
SIZE: 1068 amino acids; 124284 Da
SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).
DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
Species:
Human
Molecular Weight:
p110α (125.3kDa) & p85α (83.7kDa)
Brand Family:
Upstate
Source:
Coexpressed by baculovirus in Sf21 insect cells
Protein Target:
PI3Kα/p85α
Storage Conditions:
6 months at -70°C
Target Sub-Family:
PI3K/PI4K
Gene Symbol:
- PIK3CA
- PI3K
- MGC142161
- p110-alpha
- MGC142163
Product Name:
PI3 Kinase (p110α/p85α), active
Quality Assurance:
routinely evaluated by PIProfiler
UniProt Number:
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Packaging:
Also available in 250μg size as catalog # 14-602M - Call for pricing and availability.
Entrez Gene Number:
Purification Method:
Ni2+/NTA-agarose