Ordering Information
- : 14-603
- : 20 µg
Product Images
Sample enzyme activity data. See certificate of analysis for lot specific activity data.
PI3 Kinase (p110β/p85α) Protein, active, 20 μg
Description:
PI3 Kinase (p110β/p85α), active
Trade Name:
Upstate (Millipore)
Qty/Pk:
20 µg
Product Overview:
Complex of N-terminal His6-tagged recombinant full-length human p110β and untagged, recombinant, full length, human p85&alpha
Applications:
20 µg of PI3 Kinase (p110β/p85α). Active complex of N-terminal His6-tagged recombinant full-length human p110β and untagged, recombinant, full length, human p85&alpha. For use in Kinase Assays.
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. They have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kD catalytic subunit, such as PIK3CB, and an 85-kD adaptor subunit (Hu et al., 1993 [PubMed 8246984]).[supplied by OMIM]
UniProt Summary:
FUNCTION: SwissProt: P42338 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
SIZE: 1070 amino acids; 122762 Da
SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit.
TISSUE SPECIFICITY: Expressed ubiquitously.
SIMILARITY: SwissProt: P42338 ## Belongs to the PI3/PI4-kinase family. & Contains 1 PI3K/PI4K domain.
SIZE: 1070 amino acids; 122762 Da
SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit.
TISSUE SPECIFICITY: Expressed ubiquitously.
SIMILARITY: SwissProt: P42338 ## Belongs to the PI3/PI4-kinase family. & Contains 1 PI3K/PI4K domain.
Species:
Human
Molecular Weight:
p110β (124kDa) & p85&alpha (83.7kDa)
Brand Family:
Upstate
Source:
Coexpressed by baculovirus in Sf21 insect cells
Protein Target:
PI3Kβ/p85α
Storage Conditions:
6 months at -70°C
Target Sub-Family:
PI3K/PI4K
Promotional Text:
This enzyme is part of KinaseProfiler™, a custom inhibitor selectivity discovery service.
Gene Symbol:
- PIK3CB
- DKFZp779K1237
- PI3K
- p110-BETA
- PI3Kbeta
- MGC133043
- PIK3C1
Product Name:
PI3 Kinase (p110β/p85α), active
Quality Assurance:
routinely evaluated in a 30 minute reaction using the PIProfiler™ protocol (see www.upstate.com/features/piprofiler).
UniProt Number:
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Packaging:
Also available in 250μg size as catalog # 14-603M - Call for pricing and availability.
Entrez Gene Number:
Purification Method:
Ni2+/NTA-agarose