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Sample enzyme activity data. See certificate of analysis for lot specific activity data.
ASK1, active
Description:
ASK1, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
10 µg
Product Overview:
N-Terminal GST and C-terminal His6-tagged, recombinant, human ASK1 amino acids 649-946
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
UniProt Summary:
FUNCTION: SwissProt: Q99683 # Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
COFACTOR: Magnesium.
SIZE: 1374 amino acids; 154537 Da
SUBUNIT: Homodimer when inactive. Binds both upstream activators and downstream substrates in multimolecular complexes. Associates with and inhibited by HIV-1 Nef. Interacts with DAB2IP.
TISSUE SPECIFICITY: Abundantly expressed in heart and pancreas.
SIMILARITY: SwissProt: Q99683 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. & Contains 1 protein kinase domain.
COFACTOR: Magnesium.
SIZE: 1374 amino acids; 154537 Da
SUBUNIT: Homodimer when inactive. Binds both upstream activators and downstream substrates in multimolecular complexes. Associates with and inhibited by HIV-1 Nef. Interacts with DAB2IP.
TISSUE SPECIFICITY: Abundantly expressed in heart and pancreas.
SIMILARITY: SwissProt: Q99683 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. & Contains 1 protein kinase domain.
Species:
Human
Molecular Weight:
61.5kDa
Quality Assurance:
routinely evaluated by phosphorylation of MBP
Brand Family:
Upstate
Source:
expressed in E. coli cells
Protein Target:
Ask1
UniProt Number:
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Target Sub-Family:
STE
Storage Conditions:
6 months at -70°C
Promotional Text:
This enzyme is part of KinaseProfiler™, a custom inhibitor selectivity discovery service.
Packaging:
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-606M when ordering the 250 µg size.
Gene Symbol:
- MAP3K5
- MEKK5
- ASK1
- ASK-1
- MAPKKK5
Protein or Enzyme Type:
Kinases
Product Name:
ASK1, active
Entrez Gene Number:
Purification Method:
Ni2+/NTA-agarose