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Sample enzyme activity data. See certificate of analysis for lot specific activity data.
c-Kit (D816V), active
Description:
c-Kit (D816V), active
Trade Name:
Upstate (Millipore)
Qty/Pk:
10 µg
Product Overview:
N-terminal GST tagged, recombinant human c-Kit, amino acids 544-end containing the D816V mutation
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Summary:
FUNCTION: SwissProt: P10721 # This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K).
SIZE: 976 amino acids; 109865 Da
SUBUNIT: Interacts with APS. Interacts with the tenth PDZ domain of MPDZ.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DISEASE: SwissProt: P10721 # Defects in KIT are a cause of piebaldism [MIM:172800]. Piebaldism is an autosomal dominant genetic developmental abnormality of pigmentation characterized by congenital patches of white skin and hair that lack melanocytes. & Defects in KIT are a cause of gastrointestinal stromal tumor (GIST) [MIM:606764]. & Defects in KIT have been associated with testicular tumors [MIM:273300]. It includes germ cell tumor (GCT) or testicular germ cell tumor (TGCT).
SIMILARITY: SwissProt: P10721 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
SIZE: 976 amino acids; 109865 Da
SUBUNIT: Interacts with APS. Interacts with the tenth PDZ domain of MPDZ.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DISEASE: SwissProt: P10721 # Defects in KIT are a cause of piebaldism [MIM:172800]. Piebaldism is an autosomal dominant genetic developmental abnormality of pigmentation characterized by congenital patches of white skin and hair that lack melanocytes. & Defects in KIT are a cause of gastrointestinal stromal tumor (GIST) [MIM:606764]. & Defects in KIT have been associated with testicular tumors [MIM:273300]. It includes germ cell tumor (GCT) or testicular germ cell tumor (TGCT).
SIMILARITY: SwissProt: P10721 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
Species:
Human
Molecular Weight:
76.7kDa
Quality Assurance:
routinely evaluated by phosphorylation of 0.1mg/ml poly (Glu, Tyr)
Brand Family:
Upstate
Source:
Expressed in Sf21 insect cells
Protein Target:
c-Kit
UniProt Number:
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme
Target Sub-Family:
TK
Storage Conditions:
6 months at -70°C
Promotional Text:
This enzyme is part of KinaseProfiler™, a custom inhibitor selectivity discovery service.
Packaging:
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-611M when ordering the 250 µg size.
Gene Symbol:
- KIT
- C-Kit
- PBT
- CD117
- SCFR
- c-kit
Protein or Enzyme Type:
Kinases
Product Name:
c-Kit (D816V), active
Entrez Gene Number:
Purification Method:
glutathione agarose