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CaM Kinase I, active
Description:
CaM Kinase I, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
10 µg
Product Overview:
N-terminal GST-tagged, recombinant, human CaMK1, amino acids 2-end.
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase.
UniProt Summary:
FUNCTION: SwissProt: Q14012 # Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes like transcriptional regulation, hormone production, translational regulation, regulation of actin filament organization and neurite outgrowth. Involved in calcium- dependent activation of the ERK pathway (By similarity). Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]- x(3)-[MVLIF]. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CTFR, MYL9, SYN1/synapsin I and SYNII/synapsin II (By similarity).
SIZE: 370 amino acids; 41337 Da
SUBUNIT: Monomer. Interacts with XPO1 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note=Predominantly cytoplasmic (By similarity).
TISSUE SPECIFICITY: Ubiquitous.
DOMAIN:SwissProt: Q14012 The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate (By similarity).
SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. & Contains 1 protein kinase domain.
SIZE: 370 amino acids; 41337 Da
SUBUNIT: Monomer. Interacts with XPO1 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note=Predominantly cytoplasmic (By similarity).
TISSUE SPECIFICITY: Ubiquitous.
DOMAIN:SwissProt: Q14012 The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate (By similarity).
SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. & Contains 1 protein kinase domain.
Species:
Human
Molecular Weight:
68 kDa
Quality Assurance:
Routinely evaluated by phosphorylation of ZIPtide (#12-545).
Brand Family:
Upstate
Source:
Expressed in E. coli cells
Presentation:
50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 50% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Protein Target:
CaMKI
UniProt Number:
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Storage Conditions:
6 months at -20°C
Target Sub-Family:
CAMK
Gene Symbol:
- CAMK1
- CaMKI-alpha
- MGC120318
- CAMK1-PEN
- CAMKI
- CaM-KI
- CaMKI
- MGC120317
Protein or Enzyme Type:
Kinases
Product Name:
CaM Kinase I, active
Entrez Gene Number:
Purification Method:
Glutathione-agarose