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Ordering Information
- : 14-731
- : 20 µg
- : For concentration, please refer to the Certificate of Analysis for individual lots of this enzyme.
CaM Kinase Iδ, active
Description:
CaM Kinase Iδ, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
20 µg
Product Overview:
N-terminal 6His-tagged, recombinant, human, full length CaM Kinase Iδ.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a member of the Ca2+/calmodulin-dependent protein kinase 1 subfamily of serine/threonine kinases. The encoded protein may be involved in the regulation of granulocyte function through the chemokine signal transduction pathway. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.
UniProt Summary:
FUNCTION: SwissProt: Q8IU85 # Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May regulate calcium-mediated granulocyte function. May play a role in apoptosis of erythroleukemia cells. Activates MAP kinase MAPK3 (By similarity). In vitro, phosphorylates transcription factor CREM isoform Beta and probably CREB1.
SIZE: 385 amino acids; 42914 Da
SUBCELLULAR LOCATION: Cytoplasm (Probable). Nucleus (Probable). Note=Predominantly cytoplasmic (Probable). Also nuclear upon activation (Probable).
TISSUE SPECIFICITY: Broadly expressed. Highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes.
DOMAIN:SwissProt: Q8IU85 The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate (By similarity).
SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. & Contains 1 protein kinase domain.
SIZE: 385 amino acids; 42914 Da
SUBCELLULAR LOCATION: Cytoplasm (Probable). Nucleus (Probable). Note=Predominantly cytoplasmic (Probable). Also nuclear upon activation (Probable).
TISSUE SPECIFICITY: Broadly expressed. Highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes.
DOMAIN:SwissProt: Q8IU85 The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate (By similarity).
SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. & Contains 1 protein kinase domain.
Species:
Human
Molecular Weight:
46.7kDa
Quality Assurance:
Routinely evaluated by phosphorylation of 150μM KKLRRTLSVA.
Brand Family:
Upstate
Source:
Recombinant human protein expressed by baculovirus in Sf21 cells.
Presentation:
Presented as a frozen liquid in 50mM Tris-HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Protein Target:
CaMKIδ
UniProt Number:
Specific Activity:
For Specific Activity data, please refer to the Certificate of Analysis for individual lots of this enzyme.
Target Sub-Family:
CAMK
Storage Conditions:
Stable for 6 months at -70°C from date of shipment. Upon first use, rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70deg;C.
Promotional Text:
This enzyme is part of KinaseProfiler™, a custom inhibitor selectivity discovery service.
Gene Symbol:
- CAMK1D
- CaM-K1
- CaMKI-delta
- Camk1D
- CKLiK
- OTTHUMP00000045030
- CaMKID
Protein or Enzyme Type:
Kinases
Product Name:
CaM Kinase Iδ, active
Entrez Gene Number:
Alternate Names:
Calcium/calmodulin-dependent protein kinase type 1D; CaMKI-delta
Concentration:
For concentration, please refer to the Certificate of Analysis for individual lots of this enzyme.
Purity:
For purity information, please refer to the Certificate of Analysis for individual lots of this enzyme.
Purification Method:
Ni2+/NTA-agarose