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Abl (H396P), active
Description:
Abl (H396P), active
Trade Name:
Upstate (Millipore)
Qty/Pk:
10 µg
Product Overview:
The Abl tyrosine kinase inhibitor STI571 (Gleevec™) is an effective therapy for stable-phase chronic myeloid leukemia patients. Many patients responding to STI571 later relapse, due to a reactivation of Bcr-Abl activity. In certain cases this appears to correlate with the presence of the H396P mutation, which confers resistance to drug binding due to steric interference. (Corbin AS. et al, Blood, (2002); 101:4611-4614 and La Rosee P. et al, Cancer Research; 62:7149-7153).
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Molecular Weight:
121 kDa
Quality Assurance:
Routinely evaluated by phosphorylation of Abltide.
Brand Family:
Upstate
Source:
N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the H396P mutation. Expressed by baculovirus in Sf21 insect cells.
Presentation:
50mM Tris/HCl pH7.5, 150mM NaCl, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij 35. Frozen solution.
Protein Target:
Abl
UniProt Number:
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Storage Conditions:
Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Target Sub-Family:
TK
Promotional Text:
This enzyme is part of KinaseProfiler™, a custom inhibitor selectivity discovery service.
Packaging:
Also available in 250μg size as catalog # 14-750M - Call for pricing and availability.
Gene Symbol:
- ABL1
- JTK7
- ABL
- p150
- vABL
- cABL
- bcr/abl
Protein or Enzyme Type:
- Kinases
- Signal Transduction
- Recombinant Protein
Product Name:
Abl (H396P), active
Entrez Gene Number:
Purification Method:
Ni2+/NTA-agarose