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ACK1, active
Description:
ACK1, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
10 μg
Product Overview:
N-terminal GST-tagged recombinant, human ACK1, residues 1-389.
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.
UniProt Summary:
FUNCTION: SwissProt: Q07912 # Downstream effector of CDC42 which mediates CDC42- dependent cell migration via phosphorylation of BCAR1. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. Participates in clathrin-mediated endocytosis. May be involved both in adult synaptic function and plasticity and in brain development.
COFACTOR: Magnesium.
SIZE: 1036 amino acids; 114327 Da
SUBUNIT: Interacts with CDC42. Interacts with activated CSPG4.
SUBCELLULAR LOCATION: Cell membrane (By similarity).
SIMILARITY: SwissProt: Q07912 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. & Contains 1 CRIB domain. & Contains 1 protein kinase domain. & Contains 1 SH3 domain.
COFACTOR: Magnesium.
SIZE: 1036 amino acids; 114327 Da
SUBUNIT: Interacts with CDC42. Interacts with activated CSPG4.
SUBCELLULAR LOCATION: Cell membrane (By similarity).
SIMILARITY: SwissProt: Q07912 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. & Contains 1 CRIB domain. & Contains 1 protein kinase domain. & Contains 1 SH3 domain.
Species:
Human
Molecular Weight:
71.5 kDa
Brand Family:
Upstate
Source:
Expressed by baculovirus in Sf21 insect cells.
Protein Target:
ACK1
UniProt Number:
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Target Sub-Family:
TK
Storage Conditions:
-70°C from date of shipment.
Promotional Text:
This enzyme is part of KinaseProfiler™, a custom inhibitor selectivity discovery service.
Packaging:
Also available in 250 μg size as catalog # 14-6756M - Call for pricing and availability.
Gene Symbol:
- TNK2
- FLJ44758
- ACK1
- p21cdc42Hs
- ACK
- ACK-1
- FLJ45547
Protein or Enzyme Type:
Kinases
Product Name:
ACK1, active
Entrez Gene Number:
Alternate Names:
TNK2; ACK; FLJ44758; FLJ45547; p21cdc42Hs; tyrosine kinase, non-receptor, 2
Purification Method:
Glutathione-agarose