PI3 Kinase (p110α (E542K)/p85α)
Description:
PI 3-Kinase (p110α(E542K)/p85α), Human
Product Overview:
Complex of N-terminal 6His-tagged, recombinant, full-length human p110α containing the mutation E542K and untagged, recombinant, full-length human p85α.
Background Information:
The E542K substitution is a somatic mutation in p110α that has been associated with tumours of the colon and brain. Combined in vitro and in vivo studies have shown that this mutation confers higher lipid kinase activity than wild type, and is able to induce oncogenic transformation.
Packaging:
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-782M when ordering the 250 µg size.
Purification Method:
Ni2+ /NTA agarose
Gene Symbol:
- PIK3CA
- PI3K
- MGC142161
- p110-alpha
- MGC142163
Protein Type:
Kinase
Molecular Weight:
p110α (E542K) MW = 125.3kDa, p85α MW = 83.7kDa.
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Protein:
PI3 Kinase
Storage Conditions:
Store at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Species:
Human
Source:
Coexpressed by baculovirus in Sf21 insect cells.