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IC50 determination for Rapamycin in the presence of 10 mM FKBP12.
mTOR and FKBP12
Description:
mTOR and FKBP12
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Product Overview:
This product includes 10 µg of active mTOR (1362-end) and 1 mg of FKBP12.
Background Information:
FKBP12: N-terminal 6His-tagged recombinant, full length, human FKBP12, expressed in E.coli.
mTOR (1362-end), active: N-terminal FLAG™-tagged, recombinant, human mTOR, amino acids 1362-end, expressed by baculovirus in Sf21 insect cells.
mTOR (1362-end), active: N-terminal FLAG™-tagged, recombinant, human mTOR, amino acids 1362-end, expressed by baculovirus in Sf21 insect cells.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The CDT6 gene is located in an intron of this gene.
UniProt Summary:
FUNCTION: SwissProt: P42345 # Acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.
SIZE: 2549 amino acids; 288892 Da
SUBUNIT: Interacts with the FKBP12-rapamycin complex. Binds UBQLN1. Forms part of the mammalian target of rapamycin 2 complex (TORC2) comprised of FRAP1, GBL, PRR5, RICTOR and SIN. TORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to PRR5 and RICTOR within the TORC2 complex.
TISSUE SPECIFICITY: Expressed in numerous tissues, with highest levels in testis.
SIMILARITY: SwissProt: P42345 ## Belongs to the PI3/PI4-kinase family. & Contains 1 FAT domain. & Contains 1 FATC domain. & Contains 7 HEAT repeats. & Contains 1 PI3K/PI4K domain.
SIZE: 2549 amino acids; 288892 Da
SUBUNIT: Interacts with the FKBP12-rapamycin complex. Binds UBQLN1. Forms part of the mammalian target of rapamycin 2 complex (TORC2) comprised of FRAP1, GBL, PRR5, RICTOR and SIN. TORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to PRR5 and RICTOR within the TORC2 complex.
TISSUE SPECIFICITY: Expressed in numerous tissues, with highest levels in testis.
SIMILARITY: SwissProt: P42345 ## Belongs to the PI3/PI4-kinase family. & Contains 1 FAT domain. & Contains 1 FATC domain. & Contains 7 HEAT repeats. & Contains 1 PI3K/PI4K domain.
Species:
Human
Components:
- mTOR (1362-end), active (Cat.# 14-770)
- FKBP12
Brand Family:
Upstate
Presentation:
mTOR Enzyme in 10mM HEPES, 50mM NaCl, 50mM β-glycerophosphate, 0.25 mM sodium orthovanadate, 10 mM NaF, 300 μg/ml FLAG™ peptide, 20% v/v glycerol.
FKBP12in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
FKBP12in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Protein Target:
mTOR (FRAP1)
UniProt Number:
Storage Conditions:
Store -70°C
Target Sub-Family:
Lipid/Atypical
Gene Symbol:
- FRAP1
- RAPT1
- MTOR
- FRAP
- mTOR
- FLJ44809
- FRAP2
- RAFT1
Product Name:
mTOR and FKBP12
Entrez Gene Number: