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Sample enzyme activity data. See certificate of analysis for lot specific activity data.
PI3 Kinase (p110α/p65α) Murine
Description:
PI3 Kinase (p110α/p65α) Murine
Trade Name:
Upstate (Millipore)
Qty/Pk:
10 μg
Product Overview:
Complex of N-terminal 6His-tagged, recombinant, full-length mouse p110α and untagged, recombinant, full length mouse p65α.
Background Information:
p65α is an oncogenic mutant form of p85α(residues 1-571) which leads to increased PI3K activity.
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
UniProt Summary:
FUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
SIZE: 1068 amino acids; 124284 Da
SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).
DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
SIZE: 1068 amino acids; 124284 Da
SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).
DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
Species:
Mouse
Molecular Weight:
p110α MW = 129kDa, p65α MW = 65.9kDa
Brand Family:
Upstate
Source:
Coexpressed by baculovirus in Sf21 insect cells
Protein Target:
PI3Kα/p65α
UniProt Number:
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Target Sub-Family:
PI3K/PI4K
Storage Conditions:
Store at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap
Packaging:
Also available in 250μg size --call for pricing and availability and reference catalog number 14-786M when ordering the 250μg size.
Protein/Isoform Description:
Phosphoinositol-3-kinases (PI3 Kinases, PI3K) are a family of lipid kinases that mediate many intracellular signaling responses in both physiological and pathophysiological states. PI3 Kinase is a heteroduplex with three classes (I, II, & III) of catalytic domain. The class 1 PI3 Kinases are made up of a p85 regulatory protein and a p110 catalytic domain. There are 4 isoforms of the class 1 p110 catalytic domains. Class 1(A) include a, b, g and class 1(B) d. They are activated downstream via receptor tyrosine kinases or G-protein coupled receptors. Once activated, PI3Ks generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) from PIP2. It was later found that PTEN, a lipid phosphatase, acts opposite to PI3K by dephosphorylating PIP3, converting it to PIP2, thus deactivating the effects of PI3K. Increased levels of PIP3 in the cell leads to the activation of many key signaling regulators including Akt and PDK as well as calcium release. One of the primary roles of PI3K is its regulation of cell growth and cell metabolism. Both of these play a critical role in cancer as some believe that a tumor’s shift to glycolysis is due to the activation of PI3K. PI 3-kinases have been linked with numerous disease states, including cancer, diabetes, allergic response, hypertension, cardiac contractility, atherosclerosis, sepsis and autoimmune/ inflammatory disorders.
Gene Symbol:
- PIK3CA
- PI3K
- MGC142161
- p110-alpha
- MGC142163
Protein or Enzyme Type:
Kinases
Product Name:
PI3 Kinase (p110α/p65α) Murine
Entrez Gene Number:
Purification Method:
Ni2+/NTA-agarose