PI3 Kinase (p110β/p85β) Murine

PI3 Kinase (p110β/p85β) Murine

Upstate (Millipore)

10 μg

Complex of N-terminal 6His-tagged, recombinant, full-length mouse p110β and untagged, recombinant, full length mouse p85β.

Kinase Assay

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. They have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kD catalytic subunit, such as PIK3CB, and an 85-kD adaptor subunit (Hu et al., 1993 [PubMed 8246984]).[supplied by OMIM]

FUNCTION: SwissProt: P42338 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
SIZE: 1070 amino acids; 122762 Da
SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit.
TISSUE SPECIFICITY: Expressed ubiquitously.
SIMILARITY: SwissProt: P42338 ## Belongs to the PI3/PI4-kinase family. & Contains 1 PI3K/PI4K domain.

Mouse

p110β MW = 125.5kDa, p85β MW = 81.4kDa

Upstate

Coexpressed by baculovirus in Sf21 insect cells.

PI3Kβ/p85β

P42338

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

PI3K/PI4K

Store at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.

This enzyme is part of KinaseProfiler™, a custom inhibitor selectivity discovery service.

Also available in 250μg size --call for pricing and availability and reference catalog number 14-788M when ordering the 250μg size.

Phosphoinositol-3-kinases (PI3 Kinases, PI3K) are a family of lipid kinases that mediate many intracellular signaling responses in both physiological and pathophysiological states. PI3 Kinase is a heteroduplex with three classes (I, II, & III) of catalytic domain. The class 1 PI3 Kinases are made up of a p85 regulatory protein and a p110 catalytic domain. There are 4 isoforms of the class 1 p110 catalytic domains. Class 1(A) include a, b, g and class 1(B) d. They are activated downstream via receptor tyrosine kinases or G-protein coupled receptors. Once activated, PI3Ks generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) from PIP2. It was later found that PTEN, a lipid phosphatase, acts opposite to PI3K by dephosphorylating PIP3, converting it to PIP2, thus deactivating the effects of PI3K. Increased levels of PIP3 in the cell leads to the activation of many key signaling regulators including Akt and PDK as well as calcium release. One of the primary roles of PI3K is its regulation of cell growth and cell metabolism. Both of these play a critical role in cancer as some believe that a tumor’s shift to glycolysis is due to the activation of PI3K. PI 3-kinases have been linked with numerous disease states, including cancer, diabetes, allergic response, hypertension, cardiac contractility, atherosclerosis, sepsis and autoimmune/ inflammatory disorders.

• PIK3CB
• DKFZp779K1237
• PI3K
• p110-BETA
• PI3Kbeta
• MGC133043
• PIK3C1

Kinases

PI3 Kinase (p110β/p85β) Murine

NM_006219.1

Ni²⁺/NTA-agarose