PI 3-Kinase (p110α(E542K)/p85α) murine
Description:
PI 3-Kinase (p110α(E542K)/p85α) murine
Product Overview:
Complex of N-terminal 6His-tagged recombinant murine p110α full length, containing the mutation E542K, and untagged, recombinant, murine p85α full length.
Background Information:
The E542K substitution is a somatic mutation in p110α that has been associated with tumours of the colon and brain. Combined in vitro and in vivo studies have shown that this mutation confers higher lipid kinase activity than wild type, and is able to induce oncogenic transformation. (Kang S. et al., PNAS, (2005);102: 802-807 and Zhao J.J. et al., PNAS, (2005);102:18443-18448).
Packaging:
Also available in 250µg size --call for pricing and availability and reference catalog number 14-791M when ordering the 250µg size.
Purification Method:
Ni2+/NTA agarose
Protein Type:
Kinase
Molecular Weight:
p110α(E542K) MW = 129 kDa, p85α MW = 84 kDa.
Presentation:
50 mM Tris/HCl pH7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 0.2 mM PMSF, 1 mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
Protein or Enzyme Type:
Kinase
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Protein:
PI3 Kinase
Storage Conditions:
Store at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Source:
Co-expressed by baculovirus in Sf21 insect cells.