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PIP5K1γ, active
Description:
PIP5K1γ, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
10 µg
Product Overview:
N-terminal 6His-tagged, recombinant human PIP5K1γ, full length.
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a member of the type I phosphatidylinositol-4-phosphate 5-kinase family of enzymes. A similar protein in mice is found in synapses and focal adhesion plaques, and binds the FERM domain of talin through its C-terminus.
UniProt Summary:
FUNCTION: Plays a role in membrane ruffling and assembly of clathrin-coated pits at the synapse. Mediates RAC1-dependent reorganization of actin filaments. Participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.
Enzyme regulation: Activated by interaction with TLN2.
SUBUNIT STRUCTURE: Interacts with TLN1 and CSK. Interacts with TLN2 and ARF6. Interacts with AP2B1. Interacts with AP2M1; phosphorylation of PIP5K1C by CSK disrupts the interaction; clathrin competes with PIP5K1C.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Cytoplasmic, associated with the plasma membrane. Detected in focal adhesion plaques, membrane ruffles and plasma membrane invaginations.
INVOLVEMENT IN DISEASE: Defects in PIP5K1C are the cause of lethal congenital contracture syndrome type 3 (LCCS3) [MIM:611369]; also known as multiple contractural syndrome Israeli Bedouin type B. LCCS is an autosomal recessive disorder characterized by early fetal hydrops and akinesia, the Pena-Shokeir phenotype, specific neuropathology with degeneration of anterior horn neurons and extreme skeletal muscle atrophy. LCCS3 patients present at birth with severe multiple joint contractures with severe muscle wasting and atrophy, mainly in the legs. LCCS3 can be distinguished from the original LCCS by the absence of hydrops, fractures, and multiple pterygia.
SEQUENCE SIMILARITIES: Contains 1 PIPK domain.
Enzyme regulation: Activated by interaction with TLN2.
SUBUNIT STRUCTURE: Interacts with TLN1 and CSK. Interacts with TLN2 and ARF6. Interacts with AP2B1. Interacts with AP2M1; phosphorylation of PIP5K1C by CSK disrupts the interaction; clathrin competes with PIP5K1C.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Cytoplasmic, associated with the plasma membrane. Detected in focal adhesion plaques, membrane ruffles and plasma membrane invaginations.
INVOLVEMENT IN DISEASE: Defects in PIP5K1C are the cause of lethal congenital contracture syndrome type 3 (LCCS3) [MIM:611369]; also known as multiple contractural syndrome Israeli Bedouin type B. LCCS is an autosomal recessive disorder characterized by early fetal hydrops and akinesia, the Pena-Shokeir phenotype, specific neuropathology with degeneration of anterior horn neurons and extreme skeletal muscle atrophy. LCCS3 patients present at birth with severe multiple joint contractures with severe muscle wasting and atrophy, mainly in the legs. LCCS3 can be distinguished from the original LCCS by the absence of hydrops, fractures, and multiple pterygia.
SEQUENCE SIMILARITIES: Contains 1 PIPK domain.
Species:
Human
Molecular Weight:
77kDa
Brand Family:
Upstate
Source:
Expressed by baculovirus in Sf21 insect cells
Protein Target:
PIP5K1γ
Presentation:
10µg of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
Storage Conditions:
6 months at -70°C
Target Sub-Family:
PIPK
Promotional Text:
This enzyme is part of KinaseProfiler™, a custom inhibitor selectivity discovery service.
Protein/Isoform Description:
PI 4 Kinase acts on phosphatidylinositol in the first committed step in the production of second messenger inositol-1,4,5,-trisphosphate. The generation of intracellular second messengers plays a key role in cellular signaling. In addition, it represents a mechanism to regulate interactions of the lipid bilayer with proteins and protein scaffolds involved in vesicle budding, cytoskeletal organization and signaling. Generation of inositol-1,4,5,-trisphosphate is critical as it acts as a precursor of other important phosphoinositides and has protein binding properties of its own.
Gene Symbol:
- PIP5K1C
- KIAA0589
Protein or Enzyme Type:
- Kinases
- Signal Transduction
Product Name:
PIP5K1γ, active
Quality Assurance:
Routinely evaluated in the PIProfiler.
UniProt Number:
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Packaging:
Also available in 250μg size as catalog # 14-845M - Call for pricing and availability.
Entrez Gene Number:
Purification Method:
Immobilized metal affinity chromatography (IMAC)