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PIP4K2α, active
Description:
PIP4K2α, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
10 µg
Product Overview:
N-terminal 6His-tagged, recombinant human PIP4K2α, full length.
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family.
UniProt Summary:
FUNCTION: Catalyzes the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol-4,5-biphosphate.
CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.
SUBUNIT STRUCTURE: Homodimer
TISSUE SPECIFICITY: Expressed ubiquitously, with high levels in the brain. Present in most tissues, except notably skeletal muscle and small intestine.
SIMILARITY: Contains 1 PIPK domain.
CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.
SUBUNIT STRUCTURE: Homodimer
TISSUE SPECIFICITY: Expressed ubiquitously, with high levels in the brain. Present in most tissues, except notably skeletal muscle and small intestine.
SIMILARITY: Contains 1 PIPK domain.
Species:
Human
Molecular Weight:
51kDa
Quality Assurance:
Routinely evaluated in the PIProfiler.
Brand Family:
Upstate
Source:
Expressed by baculovirus in Sf21 insect cells
Presentation:
10µg of enzyme in 50mM Tris/HCl pH8.0, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
Protein Target:
PIP4K2α
UniProt Number:
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Target Sub-Family:
PIPK
Storage Conditions:
6 months at -70°C
Promotional Text:
This enzyme is part of KinaseProfiler™, a custom inhibitor selectivity discovery service.
Packaging:
Also available in 250μg size as catalog # 14-901M - Call for pricing and availability.
Gene Symbol:
- PIP4K2A
- PIP5K2
- PIP5K2A
Protein or Enzyme Type:
- Kinases
- Signal Transduction
Product Name:
PIP4K2α, active
Entrez Gene Number:
Purification Method:
Ni2+/NTA-agarose