Ordering Information
- : 14-925
- : 10 µg
Wee1 Protein, active, 10 μg
Description:
Wee1, active
Qty/Pk:
10 µg
Product Overview:
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Applications:
10 µg of Wee1. Active, N-terminal GST-tagged and C-terminal 6His tagged, recombinant, human Wee1, amino acids 214-end. For use in Kinase Assays.
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Molecular Weight:
77 kDa
Brand Family:
Upstate
Source:
N-terminal GST-tagged and C-terminal 6His tagged, recombinant, human Wee1 amino acids 214-end expressed by baculovirus in Sf21 insect cells.
Protein Target:
WEE1
Presentation:
50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Storage Conditions:
6 months at -70°C
Target Sub-Family:
CK1
Promotional Text:
This enzyme is part of KinaseProfiler™, a custom inhibitor selectivity discovery service.
Gene Symbol:
WEE1
Product Name:
Wee1, active
Quality Assurance:
Routinely evaluated by phosphorylation of peptide LSNLYHQGKFLQTFCGSPLYRRR.
UniProt Number:
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Packaging:
Also available in 250μg size as catalog # 14-925M and in bulk as catalog # 14-925-K.
Entrez Gene Number:
Purification Method:
Purified using glutathione agarose.