Ordering Information
- : 16-103
- : 100 µg
- : 1 mg/mL
Product Images
Western Blot Analysis:
Representative lot data.
EGF treated A431 lysate was resolved by electrophoresis, transferred to PVDF membrane and pr...
Anti-Phosphotyrosine Antibody, clone 4G10®, Biotin Conjugate | 16-103
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | ICC, IP, WB | Mouse | Biotin | Monoclonal Antibody |
Description:
Anti-Phosphotyrosine Antibody, clone 4G10®, Biotin Conjugate | 16-103
Promotional Text:
Trade Name:
Upstate (Millipore)
Product Overview:
Anti-phosphotyrosine monoclonal antibody, clone 4G10 (cat.# 05-321), cross-linked to biotin.
Specificity:
Recognizes tyrosine-phosphorylated proteins from all species.
Molecular Weight:
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Epitope:
N/A
Immunogen:
Phosphotyramine-KLH.
Modifications:
Phosphorylation
Clone:
4G10®
Isotype:
IgG2bκ
Background Information:
Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in response to various treatments.
Description
Anti-phosphotyrosine monoclonal antibody, clone 4G10® (cat. # 05-321), cross-linked to biotin.
Description
Anti-phosphotyrosine monoclonal antibody, clone 4G10® (cat. # 05-321), cross-linked to biotin.
Species Reactivity:
All
Species Reactivity Note:
Human. Expected to cross-react with rat and mouse.
Application Notes:
Immunoprecipitation:
4 μg of a previous lot, used in conjunction with Streptavidin, agarose conjugate (Catalog # 16-126), immunoprecipitated phosphotyrosine containing proteins from a lysate of EGFstimulated A431 cells.
Note: To preserve phosphotyrosine, add 0.2 mM sodium orthovanadate to the lysis buffer.
Immunocytochemistry:
5-10 μg/mL of a previous lot gave positive immunostaining of EGF-stimulated A431 cells that had been fixed with ethanol:acetic acid [1:1].
Application Notes
For use in applications in which a biotin conjugate is advantageous, such as WB and IC.
4 μg of a previous lot, used in conjunction with Streptavidin, agarose conjugate (Catalog # 16-126), immunoprecipitated phosphotyrosine containing proteins from a lysate of EGFstimulated A431 cells.
Note: To preserve phosphotyrosine, add 0.2 mM sodium orthovanadate to the lysis buffer.
Immunocytochemistry:
5-10 μg/mL of a previous lot gave positive immunostaining of EGF-stimulated A431 cells that had been fixed with ethanol:acetic acid [1:1].
Application Notes
For use in applications in which a biotin conjugate is advantageous, such as WB and IC.
Control:
Pervanadate-treated human A431 cell extracts or EGF-treated human A431 cells
Quality Assurance:
Routinely evaluated by Western Blot on EGF treated A431 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected Tyrosine phosphorylated proteins on 10 μg of EGF treated A431 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected Tyrosine phosphorylated proteins on 10 μg of EGF treated A431 lysates.
Purification Method:
Protein G purfied
Presentation:
PBS containing 0.05% sodium azide.
Storage Conditions:
9 months at 2-8°C from date of shipment. NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Immunoprecipitation
- Western Blotting
Brand Family:
Upstate
Product Name:
Anti-Phosphotyrosine Antibody, clone 4G10®, Biotin Conjugate | 16-103
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µg
Format:
Biotin
Host:
Mouse