Ordering Information
- : 16-105
- : 10 blots
Product Images
WB: 20 micrograms of EGF-Stimulated A431 Cell Lysate probed with Anti-Phosphotyrosine (4G10®), HRP conjugate.
Western Blot Analysis
EGF-stimulated cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Phosphotyros...
Anti-Phosphotyrosine Antibody, clone 4G10®, HRP conjugate | 16-105
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | ELISA, WB | Mouse | HRP | Monoclonal Antibody |
Description:
Anti-Phosphotyrosine Antibody, clone 4G10®, HRP conjugate | 16-105
Promotional Text:
Trade Name:
Upstate (Millipore)
Product Overview:
Anti-Phosphotyrosine antibody (05-321), cross-linked to horseradish peroxidase
Specificity:
Recognizes tyrosine-phosphorylated proteins from all species.
Molecular Weight:
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Epitope:
N/A
Immunogen:
Phosphotyramine-KLH.
Modifications:
Phosphorylation
Clone:
4G10®
Isotype:
IgG2bκ
Background Information:
Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in response to various treatments.
Description
IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10®. Purified by Protein G-Sepharose chromatography and cross-linked to horseradish peroxidase.
Description
IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10®. Purified by Protein G-Sepharose chromatography and cross-linked to horseradish peroxidase.
Species Reactivity:
All
Species Reactivity Note:
All
Application Notes:
For use in applications in which an HRP conjugate is advantageous, such as WB and IP.
Control:
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
Quality Assurance:
Routinely evaluated in western blot on RIPA lysates from EGF-treated human A431 carcinoma cells.
Western Blot Analysis:
Tested at 0.5-2 μg/mL, with optimal performance at 2 μg/mL (10 μL per 10 mL blocking buffer). This lot detected tyrosine-phosphorylated proteins in RIPA lysates from EGF-treated human A431 carcinoma cells.
Western Blot Analysis:
Tested at 0.5-2 μg/mL, with optimal performance at 2 μg/mL (10 μL per 10 mL blocking buffer). This lot detected tyrosine-phosphorylated proteins in RIPA lysates from EGF-treated human A431 carcinoma cells.
Purification Method:
Protein G-Sepharose™ chromatography
Presentation:
200 μg of IgG2bκ-HRP conjugate in 100 μL PBS containing 0.05% ProClin®150. Sufficient for 10 western blots, if used at the optimum concentration of 2 μg/mL in 10 mL blocking buffer. Liquid.
Storage Conditions:
Stable for 6 months at 2-8°C from date of receipt. NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
NOTE: For optimal performance, remove HRP precipitate by centrifuging vial prior to use.
NOTE: For optimal performance, remove HRP precipitate by centrifuging vial prior to use.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Western Blotting
Brand Family:
Upstate
Product Name:
Anti-Phosphotyrosine Antibody, clone 4G10®, HRP conjugate | 16-105
Antibody Type:
Monoclonal Antibody
Qty/Pk:
10 blots
Format:
HRP
Host:
Mouse