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Ordering Information
- : 16-105
- : 10 blots
Product Images
WB: 20 micrograms of EGF-Stimulated A431 Cell Lysate probed with Anti-Phosphotyrosine (4G10®), HRP conjugate.
Western Blot Analysis
EGF-stimulated cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Phosphotyros...
Anti-Phosphotyrosine, clone 4G10®, HRP conjugate
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | ELISA, WB | Mouse | HRP | Monoclonal Antibody |
Description:
Anti-Phosphotyrosine, clone 4G10®, HRP conjugate
Trade Name:
Upstate (Millipore)
Product Overview:
Anti-Phosphotyrosine antibody (05-321), cross-linked to horseradish peroxidase
Specificity:
Recognizes tyrosine-phosphorylated proteins from all species.
Molecular Weight:
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Epitope:
N/A
Immunogen:
Phosphotyramine-KLH.
Modifications:
Phosphorylation
Clone:
4G10®
Isotype:
IgG2bκ
Background Information:
Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in response to various treatments.
Description
IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10®. Purified by Protein G-Sepharose chromatography and cross-linked to horseradish peroxidase.
Description
IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10®. Purified by Protein G-Sepharose chromatography and cross-linked to horseradish peroxidase.
Species Reactivity:
All
Species Reactivity Note:
All
Application Notes:
For use in applications in which an HRP conjugate is advantageous, such as WB and IP.
Control:
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
Quality Assurance:
Routinely evaluated in western blot on RIPA lysates from EGF-treated human A431 carcinoma cells.
Western Blot Analysis:
Tested at 0.5-2 μg/mL, with optimal performance at 2 μg/mL (10 μL per 10 mL blocking buffer). This lot detected tyrosine-phosphorylated proteins in RIPA lysates from EGF-treated human A431 carcinoma cells.
Western Blot Analysis:
Tested at 0.5-2 μg/mL, with optimal performance at 2 μg/mL (10 μL per 10 mL blocking buffer). This lot detected tyrosine-phosphorylated proteins in RIPA lysates from EGF-treated human A431 carcinoma cells.
Purification Method:
Protein G-Sepharose™ chromatography
Presentation:
200 μg of IgG2bκ-HRP conjugate in 100 μL PBS containing 0.05% ProClin®150. Sufficient for 10 western blots, if used at the optimum concentration of 2 μg/mL in 10 mL blocking buffer. Liquid.
Storage Conditions:
Stable for 6 months at 2-8°C from date of receipt. NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
NOTE: For optimal performance, remove HRP precipitate by centrifuging vial prior to use.
NOTE: For optimal performance, remove HRP precipitate by centrifuging vial prior to use.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Western Blotting
Brand Family:
Upstate
Protein/Isoform Description:
Protein Tyrosine phosphorylation was first described in 1979. The development of the Anti-Phosphotyrosine, clone 4G10 in 1989 was a monumental discovery for researchers. 4G10 is the first and best single monoclonal antibody for the detection of tyrosine phosphorylation. It is the most highly cited (75% of related scientific journal articles over the last three years) and validated phosphotyrosine antibody available. It consistently produces high quality, reproducible data, and has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 18 years. In addition to its unparalleled characterization and applicability, 4G10 is well known as one of the most sensitive anti-phosphotyrosines. Now Millipore is excited to announce that we have taken the sensitivity for phosphotyrosine detection to the next level. To improve on something that hundreds have tried and none have succeeded, we pooled 4G10 with the next most highly regarded Anti-phosphotyrosine, clone PY20 to make 4G10 Platinum. PY20 itself is a very poor substitute for 4G10, but its additive effect allows for a greater level of detection on more substrates that even 4G10 alone was capable of.
Product Name:
Anti-Phosphotyrosine, clone 4G10®, HRP conjugate
Antibody Type:
Monoclonal Antibody
Qty/Pk:
10 blots
Format:
HRP
Host:
Mouse