Content Loading...
Content Loading...
Product Images
IP/WB: MAP kinases were immunoprecipitated from 500 micrograms of 3T3 cell lysate with 5 micrograms of Anti-MAP Kinase 1/2 (Erk 1/2), agarose.
Anti-MAP Kinase 1/2 (Erk1/2), agarose
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, Ch | IP | Mouse | Gel Immobilized | Polyclonal Antibody |
Description:
Anti-MAP Kinase 1/2 (Erk1/2), agarose
Trade Name:
Upstate (Millipore)
Product Overview:
Anti-MAP Kinase/Erk 1/2 (06-182) covalently coupled to Protein A agarose by dimethyl-pimelimidate.
Species Reactivity:
- Human
- Mouse
- Chicken
Application Notes:
Suitable for immunoprecipitation and affinity purification.
Quality Assurance:
routinely evaluated by immunoprecipitating MAP Kinase/Erk 1/2 from a RIPA lysate of 3T3/A31 cells
Presentation:
PBS, pH 7.4 containing 0.05% sodium azide
Storage Conditions:
1 year at 4°C
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- MAPK1
- p40
- PRKM2
- MAPK2
- PRKM1
- p42-MAPK
- p38
- p41
- ERK
- ERK-2
- ERT1
- P42MAPK
- p41mapk
- ERK2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Key Applications:
Immunoprecipitation
UniProt Summary:
FUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
COFACTOR: Magnesium (By similarity).
SIZE: 360 amino acids; 41390 Da
SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4.
DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
COFACTOR: Magnesium (By similarity).
SIZE: 360 amino acids; 41390 Da
SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4.
DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
Qty/Pk:
50 µg
Brand Family:
Upstate
Format:
Gel Immobilized
Host:
Mouse
Product Name:
Anti-MAP Kinase 1/2 (Erk1/2), agarose
Antibody Type:
Polyclonal Antibody