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Anti-phospho-Ser/Thr-Pro, MPM-2, agarose conjugate
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | IC, IP | Mouse | Gel Immobilized | Monoclonal Antibody |
Description:
Anti-phospho-Ser/Thr-Pro, MPM-2, agarose conjugate
Trade Name:
Upstate (Millipore)
Product Overview:
This antibody recognizes a variety of proteins that are phosphorylated during mitosis.
MPM2 (Mitotic Protein Monoclonal #2, 05-368) cross-linked to protein G agarose by dimethylpimelimidate
MPM2 (Mitotic Protein Monoclonal #2, 05-368) cross-linked to protein G agarose by dimethylpimelimidate
Specificity:
Recognizes a phosphorylated epitope (LTPLK or YWFSPL)6,7 in a distinct class of phosphoproteins including MAP2, HSP70, cdc25, and DNA topoisomerase IIα, most of which are phosphorylated at the onset of mitosis. The number of phosphoproteins recognized by MPM2 varies from species to species and with the type of cell.
Immunogen:
Mitotic human HeLa cell cytosolic lysate.
Modifications:
Phosphorylation
Species Reactivity:
All
Species Reactivity Note:
Both animals and plants.
Application Notes:
Suitable for immunoprecipitation and affinity purification.
Quality Assurance:
routinely evaluated by immunoprecipitating mitotic MPM2 antigens from colcemid treated human HeLa cells as shown by subsequent immunoblot using the monoclonal anti-phospho-Ser/Thr-Pro, MPM2 (05-368)
Presentation:
1 mg of MPM2 antibody covalently coupled to 1 mL of protein G agarose beads and provided as a 50% slurry suspended in PBS with 0.05% sodium azide for a final volume of 2 mL.
Storage Conditions:
1 year at 4°C
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Immunoprecipitation
Qty/Pk:
1 mg
Brand Family:
Upstate
Format:
Gel Immobilized
Host:
Mouse
Protein/Isoform Description:
MPM-2 (mitotic protein monoclonal-2) is known industry wide as one of the most versatile phospho-serine/threonine antibodies available because of its ability to recognize more than 40 mitotic phosphoproteins. Upon entry into M (Mitosis) phase, many proteins are phosphorylated either directly or indirectly by M-phase-promoting factor (MPF). Mitotic Protein Monoclonal 2 is an antibody which recognizes a phospho-epitope present in a large number of proteins, primarily during G2 and M phases of the cell cycle. While no true pan-phospho-serine or -threonine antibody has ever been produced, MPM-2 recognizes a wide variety of sites, most proline-directed, and is therefore suitable for the non-radioactive detection of many phospho-serines and -threonines.
Product Name:
Anti-phospho-Ser/Thr-Pro, MPM-2, agarose conjugate
Antibody Type:
Monoclonal Antibody