Informações para Pedidos
Protein A Agarose/Salmon Sperm DNA
Description:
Protein A Agarose/Salmon Sperm DNA
Trade Name:
Upstate (Millipore)
Product Overview:
Protein A covalently bound to agarose by alkylamine linkage containing sonicated Salmon Sperm DNA. The salmon sperm DNA blocks non-specific interactions with the beads for use in chromatin IP (ChIP) assays. Note part number 16-157C present in the ChIP kits is not available separately, please purchase 16-157.
Background Information:
Protein A from Staphylococcus aureus has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein A on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.
Key Applications:
Chromatin Immunoprecipitation (ChIP)
Application Notes:
Suitable for use in chromatin immunoprecipitation (ChIPs) assays
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Quality Assurance:
Routinely evaluated in a Chromatin Immunoprecipitation by pulling down DNA cross-linked to acetylated histones; subsequent detection was performed using anti-Acetyl Histone H4, ChIPs Grade (06-866)
Kit or Assay Type:
Chromatin Immunoprecipitation Kits
Presentation:
10mM Tris-HCl, 1mM EDTA, pH 8.0 containing 0.05% sodium azide.
Storage Conditions:
1 year at 4°C
Packaging:
2.5 mL packed beads provided as a 50% gel slurry for a final volume of 5 mL.