Anti-FAK, clone 4.47, agarose conjugate

Q05397

FUNCTION: SwissProt: Q05397 # Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
SIZE: 1052 amino acids; 119233 Da
SUBUNIT: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with SHB (By similarity). Interacts with TGFB1I1.
SUBCELLULAR LOCATION: Cell junction, focal adhesion; Cytoplasmic side. Note=Constituent of focal adhesions.
TISSUE SPECIFICITY: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
DOMAIN: SwissProt: Q05397 The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL (By similarity). & The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
PTM: Phosphorylated on 6 tyrosine residues upon activation.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain. ... hide » see more »

NM_005607.3

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. ... hide » see more »

Anti-FAK, clone 4.47, agarose conjugate

• Immunoaffinity Purification
• Immunoprecipitation

Suitable for immunoprecipitation and affinity purification.

Human

PBS containing 0.05% sodium azide

2 years at 4°C

• PTK2
• pp125FAK
• FAK
• FADK
• FAK1

routinely evaluated by immunoprecipitating FAK from a mouse 3T3/A31 RIPA cell lysate, as confirmed by subsequent immunoblot analysis using anti-FAK (05-537)

Signaling

Cytoskeletal Signaling

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Upstate (Millipore)

Gel Immobilized

Mouse

Anti-FAK monoclonal antibody (05-537), covalently coupled to Protein G agarose by dimethyl-pimelimidate

Monoclonal Antibody